Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig.

Ronald Craig, Angela Kamer, Sathya P. Kallur, Miho Inoue, Dennis P. Tarnow

Research output: Contribution to journalArticle

Abstract

We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.

Original languageEnglish (US)
Pages (from-to)228-236
Number of pages9
JournalJournal of Oral Implantology
Volume32
Issue number5
DOIs
StatePublished - 2006

Fingerprint

Miniature Swine
Connective Tissue
Dental Enamel
Transplants
Periodontal Ligament
Bone and Bones
Epithelium
Connective Tissue Cells
Dental Cementum
Dental Implants
Gelatin
Osteotomy
Wound Healing
enamel matrix proteins
Theoretical Models
Light
Membranes
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface : a pilot study in the minipig. / Craig, Ronald; Kamer, Angela; Kallur, Sathya P.; Inoue, Miho; Tarnow, Dennis P.

In: Journal of Oral Implantology, Vol. 32, No. 5, 2006, p. 228-236.

Research output: Contribution to journalArticle

@article{5b2405d6ae0c47c6971790abb3a85fe1,
title = "Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig.",
abstract = "We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.",
author = "Ronald Craig and Angela Kamer and Kallur, {Sathya P.} and Miho Inoue and Tarnow, {Dennis P.}",
year = "2006",
doi = "10.1563/820.1",
language = "English (US)",
volume = "32",
pages = "228--236",
journal = "Journal of Oral Implantology",
issn = "0160-6972",
publisher = "Allen Press Inc.",
number = "5",

}

TY - JOUR

T1 - Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface

T2 - a pilot study in the minipig.

AU - Craig, Ronald

AU - Kamer, Angela

AU - Kallur, Sathya P.

AU - Inoue, Miho

AU - Tarnow, Dennis P.

PY - 2006

Y1 - 2006

N2 - We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.

AB - We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.

UR - http://www.scopus.com/inward/record.url?scp=35348846363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35348846363&partnerID=8YFLogxK

U2 - 10.1563/820.1

DO - 10.1563/820.1

M3 - Article

VL - 32

SP - 228

EP - 236

JO - Journal of Oral Implantology

JF - Journal of Oral Implantology

SN - 0160-6972

IS - 5

ER -