Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly

Sally R. Frenkel, Robert M. Clancy, John Ricci, Paul E. Di Cesare, John J. Rediske, Steven B. Abramson

Research output: Contribution to journalArticle

Abstract

Objective. The migration of cells of chondrocyte lineage is believed to play a role in cartilage growth and repair. The present study examined 1) whether chondrocytes are capable of migration in vitro; and 2) the effects of nitric oxide (NO) on chondrocyte migration, adhesion, and cytoskeletal assembly. Methods. Chondrocyte migration was evaluated by 2 assays: 1) 'centrifugal' migration within a 3-dimensional collagen matrix (dot culture); and 2) directed migration under agarose in response to bone morphogenetic protein. To assess the effects of NO, chondrocytes were treated with either exogenous NO (S-nitrosoglutathione [SNO-GSH]) or a mixture of cytokines known to induce endogenous NO production. The effects of NO on chondrocyte adhesion to fibronectin-coated surfaces, as well as on actin polymerization (determined by indirect immunofluorescence), were also examined. Results. The capacity of chondrocytes to migrate was demonstrated both by the dot culture and by agarose methods. Both SNO-GSH and endogenous NO induced by cytokines inhibited this migration. Exposure to NO also inhibited attachment of chondrocytes to fibronectin and disrupted assembly of actin filaments. These effects of SNO-GSH and cytokine-induced NO production were reversed in the presence of hemoglobin and the NO synthase inhibitor N(G)-monomethyl arginine, respectively. Conclusion. NO interferes with chondrocyte migration and attachment to fibronectin, an extracellular matrix protein, probably via effects on the actin cytoskeleton. These effects of NO may result in impairment of cartilage repair, by interfering with the extracellular matrix regulation of chondrocyte function.

Original languageEnglish (US)
Pages (from-to)1905-1912
Number of pages8
JournalArthritis and Rheumatism
Volume39
Issue number11
DOIs
StatePublished - Nov 1996

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Chondrocytes
Nitric Oxide
Fibronectins
Cytokines
Actin Cytoskeleton
Sepharose
Cartilage
S-Nitrosoglutathione
Bone Morphogenetic Proteins
Extracellular Matrix Proteins
Cell Lineage
Indirect Fluorescent Antibody Technique
Nitric Oxide Synthase
Polymerization
Extracellular Matrix
Arginine
Actins
Hemoglobins
Collagen

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Frenkel, S. R., Clancy, R. M., Ricci, J., Di Cesare, P. E., Rediske, J. J., & Abramson, S. B. (1996). Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly. Arthritis and Rheumatism, 39(11), 1905-1912. https://doi.org/10.1002/art.1780391118

Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly. / Frenkel, Sally R.; Clancy, Robert M.; Ricci, John; Di Cesare, Paul E.; Rediske, John J.; Abramson, Steven B.

In: Arthritis and Rheumatism, Vol. 39, No. 11, 11.1996, p. 1905-1912.

Research output: Contribution to journalArticle

Frenkel, SR, Clancy, RM, Ricci, J, Di Cesare, PE, Rediske, JJ & Abramson, SB 1996, 'Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly', Arthritis and Rheumatism, vol. 39, no. 11, pp. 1905-1912. https://doi.org/10.1002/art.1780391118
Frenkel, Sally R. ; Clancy, Robert M. ; Ricci, John ; Di Cesare, Paul E. ; Rediske, John J. ; Abramson, Steven B. / Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly. In: Arthritis and Rheumatism. 1996 ; Vol. 39, No. 11. pp. 1905-1912.
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