Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

Aifang Han, James K.H. Tsoi, Jukka P. Matinlinna, Yu Zhang, Zhuofan Chen

Research output: Contribution to journalArticle

Abstract

Objective: The current laboratory study was to investigate the effect of different sterilization treatments on surface characteristics of zirconia, and biofilm formation on zirconia surface after exposure to these sterilization treatments. Methods: Commercially available zirconia discs (Cerconbase, Degu-Dent, Hanau, Germany) were prepared and polished to the same value of surface roughness. The discs were treated with one of the following sterilization methods steam autoclave sterilization, dry heat sterilization, ultraviolet C (UVC) irradiation, and gamma (γ) ray irradiation. The characteristics of zirconia surfaces were evaluated by scanning electron microscopy (SEM), surface roughness, surface free energy (SFE), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) measurements. Then, Staphylococcus aureus (S.a.) and Porphyromonas gingivalis (P.g.) bacteria were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. Results: Significant modifications were detected on the colour and SFE of zirconia. The colour of zirconia samples after UVC irradiation became light yellow whilst dark brown colour was observed after gamma ray irradiation. Moreover, UVC and gamma ray irradiation increased the hydrophilicity of zirconia surface. Overall, dry heat sterilized samples showed the significantly lowest amount of bacteria growth on zirconia, while UVC and gamma ray irradiation resulted in the highest. Significance: It is evident that various sterilization methods could change the surface which contribute to different biofilm formation and colour on zirconia.

Original languageEnglish (US)
Pages (from-to)272-281
Number of pages10
JournalDental Materials
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Biofilms
Zirconia
Gamma Rays
Irradiation
Gamma rays
Color
Ultraviolet Rays
Free energy
Bacteria
In Vitro Techniques
zirconium oxide
Hot Temperature
Surface roughness
Photoelectron Spectroscopy
Porphyromonas gingivalis
Autoclaves
Steam
Hydrophilicity
Hydrophobic and Hydrophilic Interactions
X-Ray Diffraction

Keywords

  • Bacteria
  • Colour
  • Gamma ray
  • Sterilization methods
  • UV
  • Zirconia

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro. / Han, Aifang; Tsoi, James K.H.; Matinlinna, Jukka P.; Zhang, Yu; Chen, Zhuofan.

In: Dental Materials, Vol. 34, No. 2, 01.02.2018, p. 272-281.

Research output: Contribution to journalArticle

Han, Aifang ; Tsoi, James K.H. ; Matinlinna, Jukka P. ; Zhang, Yu ; Chen, Zhuofan. / Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro. In: Dental Materials. 2018 ; Vol. 34, No. 2. pp. 272-281.
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