Abstract
Objective: The current laboratory study was to investigate the effect of different sterilization treatments on surface characteristics of zirconia, and biofilm formation on zirconia surface after exposure to these sterilization treatments. Methods: Commercially available zirconia discs (Cerconbase, Degu-Dent, Hanau, Germany) were prepared and polished to the same value of surface roughness. The discs were treated with one of the following sterilization methods steam autoclave sterilization, dry heat sterilization, ultraviolet C (UVC) irradiation, and gamma (γ) ray irradiation. The characteristics of zirconia surfaces were evaluated by scanning electron microscopy (SEM), surface roughness, surface free energy (SFE), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) measurements. Then, Staphylococcus aureus (S.a.) and Porphyromonas gingivalis (P.g.) bacteria were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. Results: Significant modifications were detected on the colour and SFE of zirconia. The colour of zirconia samples after UVC irradiation became light yellow whilst dark brown colour was observed after gamma ray irradiation. Moreover, UVC and gamma ray irradiation increased the hydrophilicity of zirconia surface. Overall, dry heat sterilized samples showed the significantly lowest amount of bacteria growth on zirconia, while UVC and gamma ray irradiation resulted in the highest. Significance: It is evident that various sterilization methods could change the surface which contribute to different biofilm formation and colour on zirconia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 272-281 |
| Number of pages | 10 |
| Journal | Dental Materials |
| Volume | 34 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2018 |
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Keywords
- Bacteria
- Colour
- Gamma ray
- Sterilization methods
- UV
- Zirconia
ASJC Scopus subject areas
- Materials Science(all)
- Dentistry(all)
- Mechanics of Materials
Cite this
Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro. / Han, Aifang; Tsoi, James K.H.; Matinlinna, Jukka P.; Zhang, Yu; Chen, Zhuofan.
In: Dental Materials, Vol. 34, No. 2, 01.02.2018, p. 272-281.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro
AU - Han, Aifang
AU - Tsoi, James K.H.
AU - Matinlinna, Jukka P.
AU - Zhang, Yu
AU - Chen, Zhuofan
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Objective: The current laboratory study was to investigate the effect of different sterilization treatments on surface characteristics of zirconia, and biofilm formation on zirconia surface after exposure to these sterilization treatments. Methods: Commercially available zirconia discs (Cerconbase, Degu-Dent, Hanau, Germany) were prepared and polished to the same value of surface roughness. The discs were treated with one of the following sterilization methods steam autoclave sterilization, dry heat sterilization, ultraviolet C (UVC) irradiation, and gamma (γ) ray irradiation. The characteristics of zirconia surfaces were evaluated by scanning electron microscopy (SEM), surface roughness, surface free energy (SFE), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) measurements. Then, Staphylococcus aureus (S.a.) and Porphyromonas gingivalis (P.g.) bacteria were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. Results: Significant modifications were detected on the colour and SFE of zirconia. The colour of zirconia samples after UVC irradiation became light yellow whilst dark brown colour was observed after gamma ray irradiation. Moreover, UVC and gamma ray irradiation increased the hydrophilicity of zirconia surface. Overall, dry heat sterilized samples showed the significantly lowest amount of bacteria growth on zirconia, while UVC and gamma ray irradiation resulted in the highest. Significance: It is evident that various sterilization methods could change the surface which contribute to different biofilm formation and colour on zirconia.
AB - Objective: The current laboratory study was to investigate the effect of different sterilization treatments on surface characteristics of zirconia, and biofilm formation on zirconia surface after exposure to these sterilization treatments. Methods: Commercially available zirconia discs (Cerconbase, Degu-Dent, Hanau, Germany) were prepared and polished to the same value of surface roughness. The discs were treated with one of the following sterilization methods steam autoclave sterilization, dry heat sterilization, ultraviolet C (UVC) irradiation, and gamma (γ) ray irradiation. The characteristics of zirconia surfaces were evaluated by scanning electron microscopy (SEM), surface roughness, surface free energy (SFE), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) measurements. Then, Staphylococcus aureus (S.a.) and Porphyromonas gingivalis (P.g.) bacteria were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. Results: Significant modifications were detected on the colour and SFE of zirconia. The colour of zirconia samples after UVC irradiation became light yellow whilst dark brown colour was observed after gamma ray irradiation. Moreover, UVC and gamma ray irradiation increased the hydrophilicity of zirconia surface. Overall, dry heat sterilized samples showed the significantly lowest amount of bacteria growth on zirconia, while UVC and gamma ray irradiation resulted in the highest. Significance: It is evident that various sterilization methods could change the surface which contribute to different biofilm formation and colour on zirconia.
KW - Bacteria
KW - Colour
KW - Gamma ray
KW - Sterilization methods
KW - UV
KW - Zirconia
UR - http://www.scopus.com/inward/record.url?scp=85034864287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034864287&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2017.11.012
DO - 10.1016/j.dental.2017.11.012
M3 - Article
VL - 34
SP - 272
EP - 281
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 2
ER -