Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes

Ramez M. Ali, Lina T. Al Kury, Keun Hang Susan Yang, Anwar Qureshi, Mohanraj Rajesh, Sehamuddin Galadari, Yaroslav M. Shuba, Frank Christopher Howarth, Murat Oz

    Research output: Contribution to journalArticle

    Abstract

    Cannabidiol (CBD), a major nonpsychotropic cannabinoid found in Cannabis plant, has been shown to influence cardiovascular functions under various physiological and pathological conditions. In the present study, the effects of CBD on contractility and electrophysiological properties of rat ventricular myocytes were investigated. Video edge detection was used to measure myocyte shortening. Intracellular Ca2+ was measured in cells loaded with the Ca2+ sensitive fluorescent indicator fura-2 AM. Whole-cell patch clamp was used to measure action potential and Ca2+ currents. Radioligand binding was employed to study pharmacological characteristics of CBD binding. CBD (1μM) caused a significant decrease in the amplitudes of electrically evoked myocyte shortening and Ca2+ transients. However, the amplitudes of caffeine-evoked Ca2+ transients and the rate of recovery of electrically evoked Ca2+ transients following caffeine application were not altered. CBD (1μM) significantly decreased the duration of APs. Further studies on L-type Ca2+ channels indicated that CBD inhibits these channels with IC50 of 0.1μM in a voltage-independent manner. Radioligand studies indicated that the specific binding of [3H]Isradipine, was not altered significantly by CBD. The results suggest that CBD depresses myocyte contractility by suppressing L-type Ca2+ channels at a site different than dihydropyridine binding site and inhibits excitation-contraction coupling in cardiomyocytes.

    Original languageEnglish (US)
    Pages (from-to)290-299
    Number of pages10
    JournalCell Calcium
    Volume57
    Issue number4
    DOIs
    StatePublished - Apr 1 2015

    Fingerprint

    Cannabidiol
    Calcium Signaling
    Muscle Cells
    Caffeine
    Isradipine
    Excitation Contraction Coupling
    Cannabinoids
    Fura-2
    Cannabis
    Cardiac Myocytes
    Action Potentials
    Inhibitory Concentration 50
    Binding Sites
    Pharmacology

    Keywords

    • Calcium channels
    • Cannabidiol
    • Cannabinoid
    • Contraction
    • Intracellular calcium
    • Ventricular myocytes

    ASJC Scopus subject areas

    • Physiology
    • Molecular Biology
    • Cell Biology

    Cite this

    Ali, R. M., Al Kury, L. T., Yang, K. H. S., Qureshi, A., Rajesh, M., Galadari, S., ... Oz, M. (2015). Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes. Cell Calcium, 57(4), 290-299. https://doi.org/10.1016/j.ceca.2015.02.001

    Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes. / Ali, Ramez M.; Al Kury, Lina T.; Yang, Keun Hang Susan; Qureshi, Anwar; Rajesh, Mohanraj; Galadari, Sehamuddin; Shuba, Yaroslav M.; Howarth, Frank Christopher; Oz, Murat.

    In: Cell Calcium, Vol. 57, No. 4, 01.04.2015, p. 290-299.

    Research output: Contribution to journalArticle

    Ali, RM, Al Kury, LT, Yang, KHS, Qureshi, A, Rajesh, M, Galadari, S, Shuba, YM, Howarth, FC & Oz, M 2015, 'Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes', Cell Calcium, vol. 57, no. 4, pp. 290-299. https://doi.org/10.1016/j.ceca.2015.02.001
    Ali RM, Al Kury LT, Yang KHS, Qureshi A, Rajesh M, Galadari S et al. Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes. Cell Calcium. 2015 Apr 1;57(4):290-299. https://doi.org/10.1016/j.ceca.2015.02.001
    Ali, Ramez M. ; Al Kury, Lina T. ; Yang, Keun Hang Susan ; Qureshi, Anwar ; Rajesh, Mohanraj ; Galadari, Sehamuddin ; Shuba, Yaroslav M. ; Howarth, Frank Christopher ; Oz, Murat. / Effects of cannabidiol on contractions and calcium signaling in rat ventricular myocytes. In: Cell Calcium. 2015 ; Vol. 57, No. 4. pp. 290-299.
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