Effect of titanium implant surface nanoroughness and calcium phosphate low impregnation on bone cell activity in vitro

Vincenzo Bucci-Sabattini, Clara Cassinelli, Paulo Coelho, Alberto Minnici, Alberto Trani, David M Dohan Ehrenfest

Research output: Contribution to journalArticle

Abstract

Background and objective: In the field of bone implant surfaces, the effects of nanoscale modifications have received significant attention. In the present study, bone cell activity on 2 implant surfaces with similar microtopography but distinct chemistry and nanotopography (sandblasted/acid-etched surface as control group, and calcium phosphate (CaP) low impregnated surface (Ossean) as test group, both from Intra-Lock, Boca Raton, FL) were evaluated. Study design: The 2 surfaces were characterized by X-ray photoelectronic spectroscopy (XPS) and scanning electron microscopy (SEM) up to ×200,000 magnification. The micrometer level roughness profiles were evaluated by means of computer software. Cell adhesion, proliferation, and alkaline phosphatase activity were assessed with human SaOS-2 osteoblasts and bone mesenchymal stem cells in nonosteogenic culture conditions. Results: The XPS and SEM results showed that the Ossean surface presented low levels of CaP impregnation within the titanium oxide layer and texturization at the nanometer scale (nanoroughness) compared with the control surface. Moreover Ossean surface induced significantly higher cell differentiation levels than the control (P < .01). Conclusion: This study showed that both homogeneous nanoroughness and CaP low impregnation differently affected in vitro bone cell behavior compared with the control moderately rough surface with less texturing in the nanometer scale. However, the relative importance of nanotopography and surface chemistry in cell reactions is yet to be determined.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume109
Issue number2
DOIs
StatePublished - Feb 2010

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Titanium
Bone and Bones
Electron Scanning Microscopy
Spectrum Analysis
X-Rays
Osteoblasts
Mesenchymal Stromal Cells
Cell Adhesion
Alkaline Phosphatase
Cell Differentiation
Software
Cell Proliferation
Control Groups
Acids
calcium phosphate
In Vitro Techniques

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Dentistry(all)
  • Oral Surgery

Cite this

Effect of titanium implant surface nanoroughness and calcium phosphate low impregnation on bone cell activity in vitro. / Bucci-Sabattini, Vincenzo; Cassinelli, Clara; Coelho, Paulo; Minnici, Alberto; Trani, Alberto; Ehrenfest, David M Dohan.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 109, No. 2, 02.2010, p. 217-224.

Research output: Contribution to journalArticle

Bucci-Sabattini, Vincenzo ; Cassinelli, Clara ; Coelho, Paulo ; Minnici, Alberto ; Trani, Alberto ; Ehrenfest, David M Dohan. / Effect of titanium implant surface nanoroughness and calcium phosphate low impregnation on bone cell activity in vitro. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 2010 ; Vol. 109, No. 2. pp. 217-224.
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