Effect of TGF-beta 1 on PDGF receptors expression in human scar fibroblasts.

D. V. Messadi, A. Le, S. Berg, G. Huang, W. Zhuang, Charles Bertolami

Research output: Contribution to journalArticle

Abstract

This study examined the effect of exogenous TGF -beta1 on platelet derived growth factor alpha and beta (PDGF-alpha, beta) receptor expression in human dermal fibroblasts derived from both normal cutaneous tissues (normal skin [NSk]) and (normal scar [NSc]) and abnormal scar (keloid). TGF-beta and PDGF are present in the early phases of wound healing and are implicated in tissue fibrosis. In this study, replicate samples of NSk, NSc and keloid fibroblasts were grown to subconfluency in DMEM/10% FBS followed by replacement of media with DMEM/0.1%FBS for 24 hrs. One group of cells (NSk, NSc and keloid) were exposed to 10 ng/mL of exogenous TGF-beta1 for 24 hours, while the other group was used as control with no exposure to exogenous TGF-beta1. RadioImmunoBinding assays, Western and Northern blot analysis were performed to examine both PDGF-alpha and PDGF-beta receptor expression at the transcriptional and post-transcriptional levels. cDNA receptor probes were synthesized using polymerase chain reaction (PCR) with selected primer sets derived from published sequences. Beta-actin probe was used as a control to confirm that the same quantity of RNA was used for each experimental condition. TGF-beta1 was found to upregulate the expression of PDGF-alpha receptor for keloid fibroblasts but not for NSk or NSc fibroblasts. No effect was observed for TGF-beta 1 on PDGF-beta receptor expression for any of the cell lines examined.

Original languageEnglish (US)
JournalFrontiers in bioscience : a journal and virtual library
Volume3
StatePublished - 1998

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Platelet-Derived Growth Factor Receptors
Transforming Growth Factor beta1
Fibroblasts
Keloid
Cicatrix
Platelet-Derived Growth Factor beta Receptor
Skin
Platelet-Derived Growth Factor alpha Receptor
Tissue
Polymerase chain reaction
Transforming Growth Factor beta
Northern Blotting
Wound Healing
Actins
Assays
Fibrosis
Up-Regulation
Complementary DNA
Western Blotting
Cells

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Effect of TGF-beta 1 on PDGF receptors expression in human scar fibroblasts. / Messadi, D. V.; Le, A.; Berg, S.; Huang, G.; Zhuang, W.; Bertolami, Charles.

In: Frontiers in bioscience : a journal and virtual library, Vol. 3, 1998.

Research output: Contribution to journalArticle

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abstract = "This study examined the effect of exogenous TGF -beta1 on platelet derived growth factor alpha and beta (PDGF-alpha, beta) receptor expression in human dermal fibroblasts derived from both normal cutaneous tissues (normal skin [NSk]) and (normal scar [NSc]) and abnormal scar (keloid). TGF-beta and PDGF are present in the early phases of wound healing and are implicated in tissue fibrosis. In this study, replicate samples of NSk, NSc and keloid fibroblasts were grown to subconfluency in DMEM/10{\%} FBS followed by replacement of media with DMEM/0.1{\%}FBS for 24 hrs. One group of cells (NSk, NSc and keloid) were exposed to 10 ng/mL of exogenous TGF-beta1 for 24 hours, while the other group was used as control with no exposure to exogenous TGF-beta1. RadioImmunoBinding assays, Western and Northern blot analysis were performed to examine both PDGF-alpha and PDGF-beta receptor expression at the transcriptional and post-transcriptional levels. cDNA receptor probes were synthesized using polymerase chain reaction (PCR) with selected primer sets derived from published sequences. Beta-actin probe was used as a control to confirm that the same quantity of RNA was used for each experimental condition. TGF-beta1 was found to upregulate the expression of PDGF-alpha receptor for keloid fibroblasts but not for NSk or NSc fibroblasts. No effect was observed for TGF-beta 1 on PDGF-beta receptor expression for any of the cell lines examined.",
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AU - Le, A.

AU - Berg, S.

AU - Huang, G.

AU - Zhuang, W.

AU - Bertolami, Charles

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