Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA

Corinne L. Gibb, Wenjie Cheng, Victor N. Morozov, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

Tandem repeats of simple doublet and triplet sequences occur with high frequency in the DNA of eucaryotes. Among the most frequent is the repeat of dTG, which has unusual structural properties. We show here that HMG1 (modeled by the second HMG box motif from HMG1 of the rat, HMGb) binds to complexes formed from annealing unequal lengths of dTG·dCA and inhibits the in vitro elongation of these complexes by the Klenow fragment of DNA polymerase 1 at 37 °C. At 46 °C, HMGb enhances the elongation. Polylysine inhibits elongation at both temperatures. These results show that the stability of this repeat in vivo can be influenced by the presence of basic proteins in general, and more selectively by the abundant nuclear protein HMG1.

Original languageEnglish (US)
Pages (from-to)5418-5424
Number of pages7
JournalBiochemistry
Volume36
Issue number18
DOIs
StatePublished - May 6 1997

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HMGB1 Protein
Tandem Repeat Sequences
Nuclear Proteins
Elongation
DNA Polymerase I
Polylysine
DNA-Directed DNA Polymerase
Rats
Structural properties
Annealing
Temperature
DNA
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gibb, C. L., Cheng, W., Morozov, V. N., & Kallenbach, N. R. (1997). Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA. Biochemistry, 36(18), 5418-5424. https://doi.org/10.1021/bi962037v

Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA. / Gibb, Corinne L.; Cheng, Wenjie; Morozov, Victor N.; Kallenbach, Neville R.

In: Biochemistry, Vol. 36, No. 18, 06.05.1997, p. 5418-5424.

Research output: Contribution to journalArticle

Gibb, CL, Cheng, W, Morozov, VN & Kallenbach, NR 1997, 'Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA', Biochemistry, vol. 36, no. 18, pp. 5418-5424. https://doi.org/10.1021/bi962037v
Gibb, Corinne L. ; Cheng, Wenjie ; Morozov, Victor N. ; Kallenbach, Neville R. / Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA. In: Biochemistry. 1997 ; Vol. 36, No. 18. pp. 5418-5424.
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