Effect of glucose on Treponema denticola cell behavior

J. D. Ruby, R. Lux, W. Shi, N. W. Charon, Ananda Dasanayake

Research output: Contribution to journalArticle

Abstract

Introduction: Treponema denticola inhabits the oral subgingival environment and is part of a proteolytic benzoyl-dl-arginine-naphthylamide-positive 'red complex' associated with active periodontal disease. Spirochetes have a unique form of chemotactic motility that may contribute to their virulence. Chemotaxis is essential for efficient nutrient-directed translocation. Methods: We examined the effect of glucose on T. denticola cell velocity, expression of periplasmic flagella proteins, and chemotaxis, e.g. translocation into capillary tubes. Results: The presence of glucose did not significantly effect T. denticola cell velocity in high viscosity conditions nor did it alter periplasmic flagella protein expression. The addition of glucose to capillary tubes resulted in greater numbers of T. denticola cells in tubes containing glucose. A non-motile mutant did not migrate into capillary tubes containing glucose. Conclusion: These results are consistent with a chemotactic response to glucose that is motility dependent.

Original languageEnglish (US)
Pages (from-to)234-238
Number of pages5
JournalOral Microbiology and Immunology
Volume23
Issue number3
DOIs
StatePublished - Jun 2008

Fingerprint

Treponema denticola
Glucose
Periplasmic Proteins
Flagella
Chemotaxis
Spirochaetales
Periodontal Diseases
Viscosity
Virulence
Arginine
Food

Keywords

  • Glucose chemotaxis
  • Motility
  • Oral spirochetes
  • Periodontal diseases
  • Treponema denticola

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Effect of glucose on Treponema denticola cell behavior. / Ruby, J. D.; Lux, R.; Shi, W.; Charon, N. W.; Dasanayake, Ananda.

In: Oral Microbiology and Immunology, Vol. 23, No. 3, 06.2008, p. 234-238.

Research output: Contribution to journalArticle

Ruby, J. D. ; Lux, R. ; Shi, W. ; Charon, N. W. ; Dasanayake, Ananda. / Effect of glucose on Treponema denticola cell behavior. In: Oral Microbiology and Immunology. 2008 ; Vol. 23, No. 3. pp. 234-238.
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