Effect of amino acid substitutions in conserved residues in the leader peptide on biosynthesis of the lantibiotic mutacin II

Ping Chen, FengXia Qi, Jan Novak, Robert E. Krull, Page W. Caufield

Research output: Contribution to journalArticle

Abstract

The lantibiotic mutacin II, produced by Streptococcus mutans T8, is a ribosomally synthesized peptide antibiotic that contains thioether amino acids such as lanthionine and methyllanthionine as a result of post-translational modifications. The mutacin II leader peptide sequence shares a number of identical amino acid residues with class AII lantibiotic leader peptides. To study the role of these conservative residues in the production of active antimicrobial mutacin, 15 mutations were generated by site-directed mutagenesis. The effects of these substitutions vary from no effect to complete block-out. Mutations G-1A, G-2A, I-4D, and L-7K completely blocked the production of mature mutacin. Other mutations (I-4V, L-7M, E-8D, S-11T/A, V-12I/A, and E-13D) had no detectable effect on mutacin production. The changes of Glu-8 to Lys, Val-12 to Leu, Glu-13 to Lys reduced the mutacin production level to about 75%, 50%, and 10% of the wild-type, respectively. Thus, our data indicated that some of these conserved residues are essential for the mutacin biosynthesis, whereas others are important for optimal biosynthesis rates.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalFEMS Microbiology Letters
Volume195
Issue number2
DOIs
StatePublished - Feb 20 2001

Fingerprint

Peptide Biosynthesis
Bacteriocins
Amino Acid Substitution
Protein Sorting Signals
Mutation
Amino Acids
Streptococcus mutans
Sulfides
Post Translational Protein Processing
Site-Directed Mutagenesis
Anti-Bacterial Agents
Peptides
mutacin II

Keywords

  • Lantibiotic
  • Leader peptide
  • Mutacin
  • Mutagenesis
  • Streptococcus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Effect of amino acid substitutions in conserved residues in the leader peptide on biosynthesis of the lantibiotic mutacin II. / Chen, Ping; Qi, FengXia; Novak, Jan; Krull, Robert E.; Caufield, Page W.

In: FEMS Microbiology Letters, Vol. 195, No. 2, 20.02.2001, p. 139-144.

Research output: Contribution to journalArticle

Chen, Ping ; Qi, FengXia ; Novak, Jan ; Krull, Robert E. ; Caufield, Page W. / Effect of amino acid substitutions in conserved residues in the leader peptide on biosynthesis of the lantibiotic mutacin II. In: FEMS Microbiology Letters. 2001 ; Vol. 195, No. 2. pp. 139-144.
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