Effect of accessory proteins P19 and P20 on cytolytic activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli

Robert Manasherob, Arieh Zaritsky, Eitan Ben-Dov, Deepak Saxena, Z. Barak, Monica Einav

Research output: Contribution to journalArticle

Abstract

The gene coding for the accessory protein P19 of Bacillus thuringiensis subsp. israelensis was expressed in Escherichia coli and its product was characterized. To investigate its putative role in δ-endotoxin crystallization as a P20-like polypeptide, each of the two encoding genes, p20 and p19, was cloned for inducible expression coordinatively with cyt1Aa. The latter is known to kill its transgenic host. P20 but not P19 stabilized Cyt1Aa and protected the host cells from its lethal effect. Neither GroEL nor GroES, expressed in trans, affected Cyt1Aa as did P20. The function of P20 is thus more specific than that of the chaperones, but that of P19 remains enigmatic. The correct sequence of p19, confirmed in all five isolates of B. thuringiensis subsp. israelensis, does not explain the slow electrophoretic mobility of its 179 amino acids product.

Original languageEnglish (US)
Pages (from-to)355-364
Number of pages10
JournalCurrent Microbiology
Volume43
Issue number5
DOIs
StatePublished - 2001

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Bacillus thuringiensis
Escherichia coli
Crystallization
Endotoxins
Genes
Proteins
Amino Acids
Peptides

ASJC Scopus subject areas

  • Microbiology

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Effect of accessory proteins P19 and P20 on cytolytic activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. / Manasherob, Robert; Zaritsky, Arieh; Ben-Dov, Eitan; Saxena, Deepak; Barak, Z.; Einav, Monica.

In: Current Microbiology, Vol. 43, No. 5, 2001, p. 355-364.

Research output: Contribution to journalArticle

Manasherob, Robert ; Zaritsky, Arieh ; Ben-Dov, Eitan ; Saxena, Deepak ; Barak, Z. ; Einav, Monica. / Effect of accessory proteins P19 and P20 on cytolytic activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. In: Current Microbiology. 2001 ; Vol. 43, No. 5. pp. 355-364.
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AU - Einav, Monica

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