Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci

Simone Duarte, Hyun Koo, William Henry Bowen, Mitsue Fujimaki Hayacibara, Jaime Aparecido Cury, Masaharu Ikegaki, Pedro Luiz Rosalen

Research output: Contribution to journalArticle

Abstract

Flavonoids have been considered the main biologically active components in propolis. However, a new variety of flavonoid-free propolis was recently found and chemically classified as type 6. Because it showed activity against oral microorganisms, this study evaluated the effects of the crude ethanolic extract of this propolis and its chemical fractions on the activity of purified glucosyltransferases (GTFs) and on the growth and adherence of mutans streptococci. The inhibitory effect of propolis extracts on GTF activities was determined either in solution or adsorbed onto saliva-coated hydroxyapatite. Streptococcus mutans Ingbritt 1600, Streptococcus sobrinus 6715, and two clinical isolates of each species were used for antibacterial assays. Susceptibilities to the test extracts were analyzed using the agar diffusion method and by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC); the effect on bacterial adherence to a glass surface was also assessed. The activity of GTFs in solution was effectively inhibited by the ethanolic extract of propolis type 6 (EEP) (>80% inhibition at 0.5 mg/ml), hexane, and chloroform fractions (60-90% inhibition at 100 μg/ml); their inhibitory effects on surface enzymes were less pronounced. The EEP, hexane, and chloroform fractions also showed significant antibacterial activity. The data showed that propolis type 6 remarkably reduced GTF activity and inhibited mutans streptococci growth and adherence; these biological activities are associated with its nonpolar components.

Original languageEnglish (US)
Pages (from-to)527-531
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number4
StatePublished - Apr 2003

Fingerprint

Propolis
Glucosyltransferases
Streptococcus mutans
Growth
Hexanes
Chloroform
Flavonoids
Streptococcus sobrinus
Microbial Sensitivity Tests
Durapatite
Complex Mixtures
Saliva
Agar
Glass
Enzymes

Keywords

  • Adherence
  • Antimicrobial activity
  • Glucosyltransferase
  • Mutans streptococci
  • Propolis

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Duarte, S., Koo, H., Bowen, W. H., Hayacibara, M. F., Cury, J. A., Ikegaki, M., & Rosalen, P. L. (2003). Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci. Biological and Pharmaceutical Bulletin, 26(4), 527-531.

Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci. / Duarte, Simone; Koo, Hyun; Bowen, William Henry; Hayacibara, Mitsue Fujimaki; Cury, Jaime Aparecido; Ikegaki, Masaharu; Rosalen, Pedro Luiz.

In: Biological and Pharmaceutical Bulletin, Vol. 26, No. 4, 04.2003, p. 527-531.

Research output: Contribution to journalArticle

Duarte, S, Koo, H, Bowen, WH, Hayacibara, MF, Cury, JA, Ikegaki, M & Rosalen, PL 2003, 'Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci', Biological and Pharmaceutical Bulletin, vol. 26, no. 4, pp. 527-531.
Duarte, Simone ; Koo, Hyun ; Bowen, William Henry ; Hayacibara, Mitsue Fujimaki ; Cury, Jaime Aparecido ; Ikegaki, Masaharu ; Rosalen, Pedro Luiz. / Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci. In: Biological and Pharmaceutical Bulletin. 2003 ; Vol. 26, No. 4. pp. 527-531.
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