Early requirement of the transcriptional activator Sox9 for neural crest specification in Xenopus

Young Hoon Lee, Yoichiro Aoki, Chang Soo Hong, Natasha Saint-Germain, Christine Credidio, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

The neural crest is a multipotent population of cells that arises at the neural plate border in the vertebrate embryo. We have previously shown that a member of the Sox family of transcription factors, Sox9, is a regulator of neural crest formation in Xenopus, as Sox9-depleted embryos failed to form neural crest progenitors. Here, we describe experiments that further investigate Sox9 function during neural crest development. Induction of neural crest progenitors in Xenopus is regulated by Wnt signaling. We show that this process is largely dependent on Sox9 function as Wnt-mediated neural crest induction is inhibited in the context of Sox9-depleted embryos. Moreover, we demonstrate that Sox9 functions as a transcriptional activator during neural crest formation. Expression of a construct in which Sox9 DNA-binding domain (HMG box) is fused to the repressor domain of Drosophila engrailed blocked neural crest formation, thereby mimicking the phenotype of Sox9-depleted embryos. Finally, using a hormone-inducible inhibitory mutant of Sox9, lacking the transactivation domain, we show that Sox9 function is required for neural crest specification but not for its subsequent migration.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalDevelopmental Biology
Volume275
Issue number1
DOIs
StatePublished - Nov 1 2004

Fingerprint

Neural Crest
Xenopus
Embryonic Structures
HMG-Box Domains
Neural Plate
Transcriptional Activation
Drosophila
Vertebrates
Transcription Factors
Hormones
Phenotype
DNA

Keywords

  • Migration
  • Neural crest
  • Slug
  • Snail
  • Sox9
  • Specification
  • Wnt
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Early requirement of the transcriptional activator Sox9 for neural crest specification in Xenopus. / Lee, Young Hoon; Aoki, Yoichiro; Hong, Chang Soo; Saint-Germain, Natasha; Credidio, Christine; Saint-Jeannet, Jean-Pierre.

In: Developmental Biology, Vol. 275, No. 1, 01.11.2004, p. 93-103.

Research output: Contribution to journalArticle

Lee, Young Hoon ; Aoki, Yoichiro ; Hong, Chang Soo ; Saint-Germain, Natasha ; Credidio, Christine ; Saint-Jeannet, Jean-Pierre. / Early requirement of the transcriptional activator Sox9 for neural crest specification in Xenopus. In: Developmental Biology. 2004 ; Vol. 275, No. 1. pp. 93-103.
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