Early expression of Iε, CD23 (FcεRII), IL-4Rα, and IgE in the human fetus

Jose O. Lima, Liming Zhang, T. Prescott Atkinson, Joseph Philips, Ananda Dasanayake, Harry W. Schroeder

Research output: Contribution to journalArticle

Abstract

Background: A major predictor of childhood atopy is the concentration of IgE in the cord blood, but whether the source of cord blood IgE is maternal or fetal remains unclear. Objective: We sought to determine the pattern of in situ IgE production during ontogeny. Methods: Ninety-seven fetal, 142 natal, and 96 childhood samples were analyzed by using reverse transcription PCR for transcription of VDJCε, Iε, and CD23. Thirty-eight fetal liver samples were analyzed for the IL4RA genotype. Results: IL-4Rα, CD23a, CD23b, and sterile Iε transcripts were present as early as 8 weeks' gestation. VDJCε transcripts were found in second-trimester fetal liver and third-trimester cord blood, although they were rare. VDJCε transcripts were more common in the blood of children 9 months and older. Sequence analysis suggested that fetal VDJCε was the product of selection. All fetal livers actively transcribing Iε, VDJCε, and IL-4Rα contained at least one copy of the atopy-associated IL4RA*A1902G polymorphism. Conclusion: The human fetus contains B cells that are primed to undergo IgE class switching from the earliest stages of ontogeny and can produce endogenous IgE by 20 weeks' gestation. However, IgE-producing cells are rare until 9 months after birth.

Original languageEnglish (US)
Pages (from-to)911-917
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume106
Issue number5
DOIs
StatePublished - 2000

Fingerprint

Immunoglobulin E
Fetus
Fetal Blood
Liver
Immunoglobulin Class Switching
Pregnancy
Third Pregnancy Trimester
Second Pregnancy Trimester
Reverse Transcription
Sequence Analysis
B-Lymphocytes
Genotype
Mothers
Parturition
Polymerase Chain Reaction

Keywords

  • CD23
  • Cord blood
  • Fetus
  • Gut
  • Human
  • IgE
  • IL-4 receptor
  • Immunoglobulin variable region
  • Liver
  • Mesentery
  • Receptors Fc
  • Spleen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Early expression of Iε, CD23 (FcεRII), IL-4Rα, and IgE in the human fetus. / Lima, Jose O.; Zhang, Liming; Atkinson, T. Prescott; Philips, Joseph; Dasanayake, Ananda; Schroeder, Harry W.

In: Journal of Allergy and Clinical Immunology, Vol. 106, No. 5, 2000, p. 911-917.

Research output: Contribution to journalArticle

Lima, Jose O. ; Zhang, Liming ; Atkinson, T. Prescott ; Philips, Joseph ; Dasanayake, Ananda ; Schroeder, Harry W. / Early expression of Iε, CD23 (FcεRII), IL-4Rα, and IgE in the human fetus. In: Journal of Allergy and Clinical Immunology. 2000 ; Vol. 106, No. 5. pp. 911-917.
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AU - Dasanayake, Ananda

AU - Schroeder, Harry W.

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N2 - Background: A major predictor of childhood atopy is the concentration of IgE in the cord blood, but whether the source of cord blood IgE is maternal or fetal remains unclear. Objective: We sought to determine the pattern of in situ IgE production during ontogeny. Methods: Ninety-seven fetal, 142 natal, and 96 childhood samples were analyzed by using reverse transcription PCR for transcription of VDJCε, Iε, and CD23. Thirty-eight fetal liver samples were analyzed for the IL4RA genotype. Results: IL-4Rα, CD23a, CD23b, and sterile Iε transcripts were present as early as 8 weeks' gestation. VDJCε transcripts were found in second-trimester fetal liver and third-trimester cord blood, although they were rare. VDJCε transcripts were more common in the blood of children 9 months and older. Sequence analysis suggested that fetal VDJCε was the product of selection. All fetal livers actively transcribing Iε, VDJCε, and IL-4Rα contained at least one copy of the atopy-associated IL4RA*A1902G polymorphism. Conclusion: The human fetus contains B cells that are primed to undergo IgE class switching from the earliest stages of ontogeny and can produce endogenous IgE by 20 weeks' gestation. However, IgE-producing cells are rare until 9 months after birth.

AB - Background: A major predictor of childhood atopy is the concentration of IgE in the cord blood, but whether the source of cord blood IgE is maternal or fetal remains unclear. Objective: We sought to determine the pattern of in situ IgE production during ontogeny. Methods: Ninety-seven fetal, 142 natal, and 96 childhood samples were analyzed by using reverse transcription PCR for transcription of VDJCε, Iε, and CD23. Thirty-eight fetal liver samples were analyzed for the IL4RA genotype. Results: IL-4Rα, CD23a, CD23b, and sterile Iε transcripts were present as early as 8 weeks' gestation. VDJCε transcripts were found in second-trimester fetal liver and third-trimester cord blood, although they were rare. VDJCε transcripts were more common in the blood of children 9 months and older. Sequence analysis suggested that fetal VDJCε was the product of selection. All fetal livers actively transcribing Iε, VDJCε, and IL-4Rα contained at least one copy of the atopy-associated IL4RA*A1902G polymorphism. Conclusion: The human fetus contains B cells that are primed to undergo IgE class switching from the earliest stages of ontogeny and can produce endogenous IgE by 20 weeks' gestation. However, IgE-producing cells are rare until 9 months after birth.

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