Dynamics of spore coat morphogenesis in Bacillus subtilis

Peter T. McKenney, Patrick Eichenberger

Research output: Contribution to journalArticle

Abstract

Spores of Bacillus subtilis are encased in a protective coat made up of at least 70 proteins. The structure of the spore coat has been examined using a variety of genetic, imaging and biochemical techniques; however, the majority of these studies have focused on mature spores. In this study we use a library of 41 spore coat proteins fused to the green fluorescent protein to examine spore coat morphogenesis over the time-course of sporulation. We found considerable diversity in the localization dynamics of coat proteins and were able to establish six classes based on localization kinetics. Localization dynamics correlate well with the known transcriptional regulators of coat gene expression. Previously, we described the existence of multiple layers in the mature spore coat. Here, we find that the spore coat initially assembles a scaffold that is organized into multiple layers on one pole of the spore. The coat then encases the spore in multiple co-ordinated waves. Encasement is driven, at least partially, by transcription of coat genes and deletion of sporulation transcription factors arrests encasement. We also identify the trans-compartment SpoIIIAH-SpoIIQ channel as necessary for encasement. This is the first demonstration of a forespore contribution to spore coat morphogenesis.

Original languageEnglish (US)
Pages (from-to)245-260
Number of pages16
JournalMolecular Microbiology
Volume83
Issue number2
DOIs
StatePublished - Jan 2012

Fingerprint

Bacillus subtilis
Spores
Morphogenesis
Capsid Proteins
Gene Deletion
Regulator Genes
Green Fluorescent Proteins
Molecular Biology
Transcription Factors
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Dynamics of spore coat morphogenesis in Bacillus subtilis. / McKenney, Peter T.; Eichenberger, Patrick.

In: Molecular Microbiology, Vol. 83, No. 2, 01.2012, p. 245-260.

Research output: Contribution to journalArticle

@article{869badd0254e4896ad7674ab04e91fab,
title = "Dynamics of spore coat morphogenesis in Bacillus subtilis",
abstract = "Spores of Bacillus subtilis are encased in a protective coat made up of at least 70 proteins. The structure of the spore coat has been examined using a variety of genetic, imaging and biochemical techniques; however, the majority of these studies have focused on mature spores. In this study we use a library of 41 spore coat proteins fused to the green fluorescent protein to examine spore coat morphogenesis over the time-course of sporulation. We found considerable diversity in the localization dynamics of coat proteins and were able to establish six classes based on localization kinetics. Localization dynamics correlate well with the known transcriptional regulators of coat gene expression. Previously, we described the existence of multiple layers in the mature spore coat. Here, we find that the spore coat initially assembles a scaffold that is organized into multiple layers on one pole of the spore. The coat then encases the spore in multiple co-ordinated waves. Encasement is driven, at least partially, by transcription of coat genes and deletion of sporulation transcription factors arrests encasement. We also identify the trans-compartment SpoIIIAH-SpoIIQ channel as necessary for encasement. This is the first demonstration of a forespore contribution to spore coat morphogenesis.",
author = "McKenney, {Peter T.} and Patrick Eichenberger",
year = "2012",
month = "1",
doi = "10.1111/j.1365-2958.2011.07936.x",
language = "English (US)",
volume = "83",
pages = "245--260",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Dynamics of spore coat morphogenesis in Bacillus subtilis

AU - McKenney, Peter T.

AU - Eichenberger, Patrick

PY - 2012/1

Y1 - 2012/1

N2 - Spores of Bacillus subtilis are encased in a protective coat made up of at least 70 proteins. The structure of the spore coat has been examined using a variety of genetic, imaging and biochemical techniques; however, the majority of these studies have focused on mature spores. In this study we use a library of 41 spore coat proteins fused to the green fluorescent protein to examine spore coat morphogenesis over the time-course of sporulation. We found considerable diversity in the localization dynamics of coat proteins and were able to establish six classes based on localization kinetics. Localization dynamics correlate well with the known transcriptional regulators of coat gene expression. Previously, we described the existence of multiple layers in the mature spore coat. Here, we find that the spore coat initially assembles a scaffold that is organized into multiple layers on one pole of the spore. The coat then encases the spore in multiple co-ordinated waves. Encasement is driven, at least partially, by transcription of coat genes and deletion of sporulation transcription factors arrests encasement. We also identify the trans-compartment SpoIIIAH-SpoIIQ channel as necessary for encasement. This is the first demonstration of a forespore contribution to spore coat morphogenesis.

AB - Spores of Bacillus subtilis are encased in a protective coat made up of at least 70 proteins. The structure of the spore coat has been examined using a variety of genetic, imaging and biochemical techniques; however, the majority of these studies have focused on mature spores. In this study we use a library of 41 spore coat proteins fused to the green fluorescent protein to examine spore coat morphogenesis over the time-course of sporulation. We found considerable diversity in the localization dynamics of coat proteins and were able to establish six classes based on localization kinetics. Localization dynamics correlate well with the known transcriptional regulators of coat gene expression. Previously, we described the existence of multiple layers in the mature spore coat. Here, we find that the spore coat initially assembles a scaffold that is organized into multiple layers on one pole of the spore. The coat then encases the spore in multiple co-ordinated waves. Encasement is driven, at least partially, by transcription of coat genes and deletion of sporulation transcription factors arrests encasement. We also identify the trans-compartment SpoIIIAH-SpoIIQ channel as necessary for encasement. This is the first demonstration of a forespore contribution to spore coat morphogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84855804122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855804122&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.2011.07936.x

DO - 10.1111/j.1365-2958.2011.07936.x

M3 - Article

VL - 83

SP - 245

EP - 260

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 2

ER -