Dynamic Phenylalanine Clamp Interactions Define Single-Channel Polypeptide Translocation through the Anthrax Toxin Protective Antigen Channel

Koyel Ghosal, Jennifer M. Colby, Debasis Das, Stephen T. Joy, Paramjit Arora, Bryan A. Krantz

Research output: Contribution to journalArticle

Abstract

Anthrax toxin is an intracellularly acting toxin where sufficient detail is known about the structure of its channel, allowing for molecular investigations of translocation. The toxin is composed of three proteins, protective antigen (PA), lethal factor (LF), and edema factor (EF). The toxin's translocon, PA, translocates the large enzymes, LF and EF, across the endosomal membrane into the host cell's cytosol. Polypeptide clamps located throughout the PA channel catalyze the translocation of LF and EF. Here, we show that the central peptide clamp, the ϕ clamp, is a dynamic site that governs the overall peptide translocation pathway. Single-channel translocations of a 10-residue, guest–host peptide revealed that there were four states when peptide interacted with the channel. Two of the states had intermediate conductances of 10% and 50% of full conductance. With aromatic guest–host peptides, the 50% conducting intermediate oscillated with the fully blocked state. A Trp guest–host peptide was studied by manipulating its stereochemistry and prenucleating helix formation with a covalent linkage in the place of a hydrogen bond or hydrogen-bond surrogate (HBS). The Trp peptide synthesized with ʟ-amino acids translocated more efficiently than peptides synthesized with D- or alternating D,ʟ-amino acids. HBS stapled Trp peptide exhibited signs of steric hindrance and difficulty translocating. However, when mutant ϕ clamp (F427A) channels were tested, the HBS peptide translocated normally. Overall, peptide translocation is defined by dynamic interactions between the peptide and ϕ clamp. These dynamics require conformational flexibility, such that the peptide productively forms both extended-chain and helical states during translocation.

Original languageEnglish (US)
Pages (from-to)900-910
Number of pages11
JournalJournal of Molecular Biology
Volume429
Issue number6
DOIs
StatePublished - Mar 24 2017

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Phenylalanine
Peptides
Hydrogen
anthrax toxin
Antigens
Amino Acids
Cytosol

Keywords

  • anthrax toxin
  • Bacillus anthracis
  • electrophysiology
  • peptide clamp
  • protective antigen

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Dynamic Phenylalanine Clamp Interactions Define Single-Channel Polypeptide Translocation through the Anthrax Toxin Protective Antigen Channel. / Ghosal, Koyel; Colby, Jennifer M.; Das, Debasis; Joy, Stephen T.; Arora, Paramjit; Krantz, Bryan A.

In: Journal of Molecular Biology, Vol. 429, No. 6, 24.03.2017, p. 900-910.

Research output: Contribution to journalArticle

Ghosal, Koyel ; Colby, Jennifer M. ; Das, Debasis ; Joy, Stephen T. ; Arora, Paramjit ; Krantz, Bryan A. / Dynamic Phenylalanine Clamp Interactions Define Single-Channel Polypeptide Translocation through the Anthrax Toxin Protective Antigen Channel. In: Journal of Molecular Biology. 2017 ; Vol. 429, No. 6. pp. 900-910.
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