Dynamic microRNA gene transcription and processing during T cell development

Francis F. Kirigin, Kenneth Lindstedt, Maclean Sellars, Maria Ciofani, Siao Li Low, Lachlan Jones, Fiona Bell, Florencia Pauli, Richard Bonneau, Richard M. Myers, Dan R. Littman, Mark M W Chong

Research output: Contribution to journalArticle

Abstract

By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, and in a dynamic manner, it was unclear how comprehensive these earlier analyses were.We therefore decided to profile miRNA expression by next generation sequencing. Furthermore, we profiled miRNA expression starting from the hematopoietic stem cell. This analysis revealed that miRNA expression during T cell development is extremely dynamic, with 645 miRNAs sequenced, and the expression of some varying by as much as 3 orders of magnitude. Furthermore, changes in precursor processing led to altered mature miRNA sequences. We also analyzed the structures of the primary miRNA transcripts expressed in T cells and found that many were extremely long. The longest was pri-mir-29b-1/29a at ∼168 kb. All the long pri-miRNAs also displayed extensive splicing. Our findings indicate that miRNA expression during T cell development is both a highly dynamic and a highly regulated process.

Original languageEnglish (US)
Pages (from-to)3257-3267
Number of pages11
JournalJournal of Immunology
Volume188
Issue number7
DOIs
StatePublished - Apr 1 2012

Fingerprint

MicroRNAs
T-Lymphocytes
Genes
Critical Pathways
Hematopoietic Stem Cells
Organism Cloning

ASJC Scopus subject areas

  • Immunology

Cite this

Kirigin, F. F., Lindstedt, K., Sellars, M., Ciofani, M., Low, S. L., Jones, L., ... Chong, M. M. W. (2012). Dynamic microRNA gene transcription and processing during T cell development. Journal of Immunology, 188(7), 3257-3267. https://doi.org/10.4049/jimmunol.1103175

Dynamic microRNA gene transcription and processing during T cell development. / Kirigin, Francis F.; Lindstedt, Kenneth; Sellars, Maclean; Ciofani, Maria; Low, Siao Li; Jones, Lachlan; Bell, Fiona; Pauli, Florencia; Bonneau, Richard; Myers, Richard M.; Littman, Dan R.; Chong, Mark M W.

In: Journal of Immunology, Vol. 188, No. 7, 01.04.2012, p. 3257-3267.

Research output: Contribution to journalArticle

Kirigin, FF, Lindstedt, K, Sellars, M, Ciofani, M, Low, SL, Jones, L, Bell, F, Pauli, F, Bonneau, R, Myers, RM, Littman, DR & Chong, MMW 2012, 'Dynamic microRNA gene transcription and processing during T cell development', Journal of Immunology, vol. 188, no. 7, pp. 3257-3267. https://doi.org/10.4049/jimmunol.1103175
Kirigin FF, Lindstedt K, Sellars M, Ciofani M, Low SL, Jones L et al. Dynamic microRNA gene transcription and processing during T cell development. Journal of Immunology. 2012 Apr 1;188(7):3257-3267. https://doi.org/10.4049/jimmunol.1103175
Kirigin, Francis F. ; Lindstedt, Kenneth ; Sellars, Maclean ; Ciofani, Maria ; Low, Siao Li ; Jones, Lachlan ; Bell, Fiona ; Pauli, Florencia ; Bonneau, Richard ; Myers, Richard M. ; Littman, Dan R. ; Chong, Mark M W. / Dynamic microRNA gene transcription and processing during T cell development. In: Journal of Immunology. 2012 ; Vol. 188, No. 7. pp. 3257-3267.
@article{0cd428f9ac7f4bcbbf3a075ca708ed03,
title = "Dynamic microRNA gene transcription and processing during T cell development",
abstract = "By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, and in a dynamic manner, it was unclear how comprehensive these earlier analyses were.We therefore decided to profile miRNA expression by next generation sequencing. Furthermore, we profiled miRNA expression starting from the hematopoietic stem cell. This analysis revealed that miRNA expression during T cell development is extremely dynamic, with 645 miRNAs sequenced, and the expression of some varying by as much as 3 orders of magnitude. Furthermore, changes in precursor processing led to altered mature miRNA sequences. We also analyzed the structures of the primary miRNA transcripts expressed in T cells and found that many were extremely long. The longest was pri-mir-29b-1/29a at ∼168 kb. All the long pri-miRNAs also displayed extensive splicing. Our findings indicate that miRNA expression during T cell development is both a highly dynamic and a highly regulated process.",
author = "Kirigin, {Francis F.} and Kenneth Lindstedt and Maclean Sellars and Maria Ciofani and Low, {Siao Li} and Lachlan Jones and Fiona Bell and Florencia Pauli and Richard Bonneau and Myers, {Richard M.} and Littman, {Dan R.} and Chong, {Mark M W}",
year = "2012",
month = "4",
day = "1",
doi = "10.4049/jimmunol.1103175",
language = "English (US)",
volume = "188",
pages = "3257--3267",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Dynamic microRNA gene transcription and processing during T cell development

AU - Kirigin, Francis F.

AU - Lindstedt, Kenneth

AU - Sellars, Maclean

AU - Ciofani, Maria

AU - Low, Siao Li

AU - Jones, Lachlan

AU - Bell, Fiona

AU - Pauli, Florencia

AU - Bonneau, Richard

AU - Myers, Richard M.

AU - Littman, Dan R.

AU - Chong, Mark M W

PY - 2012/4/1

Y1 - 2012/4/1

N2 - By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, and in a dynamic manner, it was unclear how comprehensive these earlier analyses were.We therefore decided to profile miRNA expression by next generation sequencing. Furthermore, we profiled miRNA expression starting from the hematopoietic stem cell. This analysis revealed that miRNA expression during T cell development is extremely dynamic, with 645 miRNAs sequenced, and the expression of some varying by as much as 3 orders of magnitude. Furthermore, changes in precursor processing led to altered mature miRNA sequences. We also analyzed the structures of the primary miRNA transcripts expressed in T cells and found that many were extremely long. The longest was pri-mir-29b-1/29a at ∼168 kb. All the long pri-miRNAs also displayed extensive splicing. Our findings indicate that miRNA expression during T cell development is both a highly dynamic and a highly regulated process.

AB - By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, and in a dynamic manner, it was unclear how comprehensive these earlier analyses were.We therefore decided to profile miRNA expression by next generation sequencing. Furthermore, we profiled miRNA expression starting from the hematopoietic stem cell. This analysis revealed that miRNA expression during T cell development is extremely dynamic, with 645 miRNAs sequenced, and the expression of some varying by as much as 3 orders of magnitude. Furthermore, changes in precursor processing led to altered mature miRNA sequences. We also analyzed the structures of the primary miRNA transcripts expressed in T cells and found that many were extremely long. The longest was pri-mir-29b-1/29a at ∼168 kb. All the long pri-miRNAs also displayed extensive splicing. Our findings indicate that miRNA expression during T cell development is both a highly dynamic and a highly regulated process.

UR - http://www.scopus.com/inward/record.url?scp=84859403817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859403817&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.1103175

DO - 10.4049/jimmunol.1103175

M3 - Article

VL - 188

SP - 3257

EP - 3267

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -