Duplication of the Brassica oleracea APETALA1 floral homeotic gene and the evolution of domesticated cauliflower

A. C. Lowman, M. D. Purugganan

Research output: Contribution to journalArticle

Abstract

Development of the cauliflower phenotype in Arabidopsis thaliana requires mutations at both the CAULIFLOWER and APETALA1 loci. BoAP1 is the Brassica oleracea orthologue to the Arabidopsis AP1 gene, and is present in two copies in Brassica genomes. The BoAP1-A gene appears to encode a full- length protein, but BoAP1-B alleles in B. oleracea contain insertions that lead to premature translation termination. The BoAP1-B allele found in most B. oleracea subspecies, including B. oleracea ssp. botrytis (domesticated cauliflower) contains a 9 bp insertion in exon 4. This insertion leads to the formation of an in-frame translation termination codon, and these alleles can encode a protein that is truncated at the K domain of this MADS-box transcriptional activator. The allele in B. oleracea ssp. oleracea (wild cabbage) lacks this insertion and instead contains a downstream 4 bp frameshift mutation resulting in the formation of a nonsense mutation. The structure of the BoAP1-B alleles suggests that they are impaired in their ability to perform their floral meristem identity function. These mutations, in conjunction with mutations at the BoCAULIFLOWER (BoCAL) locus, may be associated with the evolution of domesticated cauliflower.

Original languageEnglish (US)
Pages (from-to)514-520
Number of pages7
JournalJournal of Heredity
Volume90
Issue number5
DOIs
StatePublished - Sep 1999

Fingerprint

homeotic genes
Homeobox Genes
Brassica
cauliflower
Brassica oleracea
alleles
Alleles
mutation
translation (genetics)
Brassica oleracea var. oleracea
Arabidopsis
Mutation
nonsense mutation
frameshift mutation
loci
Botrytis
stop codon
Frameshift Mutation
Meristem
meristems

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)

Cite this

Duplication of the Brassica oleracea APETALA1 floral homeotic gene and the evolution of domesticated cauliflower. / Lowman, A. C.; Purugganan, M. D.

In: Journal of Heredity, Vol. 90, No. 5, 09.1999, p. 514-520.

Research output: Contribution to journalArticle

@article{1033c59172de406fabc34548f95aa54f,
title = "Duplication of the Brassica oleracea APETALA1 floral homeotic gene and the evolution of domesticated cauliflower",
abstract = "Development of the cauliflower phenotype in Arabidopsis thaliana requires mutations at both the CAULIFLOWER and APETALA1 loci. BoAP1 is the Brassica oleracea orthologue to the Arabidopsis AP1 gene, and is present in two copies in Brassica genomes. The BoAP1-A gene appears to encode a full- length protein, but BoAP1-B alleles in B. oleracea contain insertions that lead to premature translation termination. The BoAP1-B allele found in most B. oleracea subspecies, including B. oleracea ssp. botrytis (domesticated cauliflower) contains a 9 bp insertion in exon 4. This insertion leads to the formation of an in-frame translation termination codon, and these alleles can encode a protein that is truncated at the K domain of this MADS-box transcriptional activator. The allele in B. oleracea ssp. oleracea (wild cabbage) lacks this insertion and instead contains a downstream 4 bp frameshift mutation resulting in the formation of a nonsense mutation. The structure of the BoAP1-B alleles suggests that they are impaired in their ability to perform their floral meristem identity function. These mutations, in conjunction with mutations at the BoCAULIFLOWER (BoCAL) locus, may be associated with the evolution of domesticated cauliflower.",
author = "Lowman, {A. C.} and Purugganan, {M. D.}",
year = "1999",
month = "9",
doi = "10.1093/jhered/90.5.514",
language = "English (US)",
volume = "90",
pages = "514--520",
journal = "Journal of Heredity",
issn = "0022-1503",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Duplication of the Brassica oleracea APETALA1 floral homeotic gene and the evolution of domesticated cauliflower

AU - Lowman, A. C.

AU - Purugganan, M. D.

PY - 1999/9

Y1 - 1999/9

N2 - Development of the cauliflower phenotype in Arabidopsis thaliana requires mutations at both the CAULIFLOWER and APETALA1 loci. BoAP1 is the Brassica oleracea orthologue to the Arabidopsis AP1 gene, and is present in two copies in Brassica genomes. The BoAP1-A gene appears to encode a full- length protein, but BoAP1-B alleles in B. oleracea contain insertions that lead to premature translation termination. The BoAP1-B allele found in most B. oleracea subspecies, including B. oleracea ssp. botrytis (domesticated cauliflower) contains a 9 bp insertion in exon 4. This insertion leads to the formation of an in-frame translation termination codon, and these alleles can encode a protein that is truncated at the K domain of this MADS-box transcriptional activator. The allele in B. oleracea ssp. oleracea (wild cabbage) lacks this insertion and instead contains a downstream 4 bp frameshift mutation resulting in the formation of a nonsense mutation. The structure of the BoAP1-B alleles suggests that they are impaired in their ability to perform their floral meristem identity function. These mutations, in conjunction with mutations at the BoCAULIFLOWER (BoCAL) locus, may be associated with the evolution of domesticated cauliflower.

AB - Development of the cauliflower phenotype in Arabidopsis thaliana requires mutations at both the CAULIFLOWER and APETALA1 loci. BoAP1 is the Brassica oleracea orthologue to the Arabidopsis AP1 gene, and is present in two copies in Brassica genomes. The BoAP1-A gene appears to encode a full- length protein, but BoAP1-B alleles in B. oleracea contain insertions that lead to premature translation termination. The BoAP1-B allele found in most B. oleracea subspecies, including B. oleracea ssp. botrytis (domesticated cauliflower) contains a 9 bp insertion in exon 4. This insertion leads to the formation of an in-frame translation termination codon, and these alleles can encode a protein that is truncated at the K domain of this MADS-box transcriptional activator. The allele in B. oleracea ssp. oleracea (wild cabbage) lacks this insertion and instead contains a downstream 4 bp frameshift mutation resulting in the formation of a nonsense mutation. The structure of the BoAP1-B alleles suggests that they are impaired in their ability to perform their floral meristem identity function. These mutations, in conjunction with mutations at the BoCAULIFLOWER (BoCAL) locus, may be associated with the evolution of domesticated cauliflower.

UR - http://www.scopus.com/inward/record.url?scp=0032745667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032745667&partnerID=8YFLogxK

U2 - 10.1093/jhered/90.5.514

DO - 10.1093/jhered/90.5.514

M3 - Article

C2 - 10544496

AN - SCOPUS:0032745667

VL - 90

SP - 514

EP - 520

JO - Journal of Heredity

JF - Journal of Heredity

SN - 0022-1503

IS - 5

ER -