Duplex DNA as a self-assembling template for the identification of bidentate protein-binding agents

Debarati M. Tagore, K. Ingrid Sprinz, Andrew Hamilton

Research output: Contribution to journalArticle

Abstract

This paper reviews the versatile self-assembly of bidentate protein-binding agents on a duplex DNA scaffold, as demonstrated using the biotin-streptavidin system. The preferential binding and stabilization of bidentate biotin-containing duplexes is reflected in a 12C increase in the melting temperature of the duplex in the presence of streptavidin.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalSupramolecular Chemistry
Volume19
Issue number1-2
DOIs
StatePublished - Jan 2007

Fingerprint

Streptavidin
Biotin
DNA
Scaffolds
Self assembly
Melting point
Stabilization
Protein Binding

Keywords

  • Bidentate
  • Duplex DNA
  • Self-assembly

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Duplex DNA as a self-assembling template for the identification of bidentate protein-binding agents. / Tagore, Debarati M.; Sprinz, K. Ingrid; Hamilton, Andrew.

In: Supramolecular Chemistry, Vol. 19, No. 1-2, 01.2007, p. 129-136.

Research output: Contribution to journalArticle

@article{4563bd9ec6684a589f0f9c253d8a7203,
title = "Duplex DNA as a self-assembling template for the identification of bidentate protein-binding agents",
abstract = "This paper reviews the versatile self-assembly of bidentate protein-binding agents on a duplex DNA scaffold, as demonstrated using the biotin-streptavidin system. The preferential binding and stabilization of bidentate biotin-containing duplexes is reflected in a 12C increase in the melting temperature of the duplex in the presence of streptavidin.",
keywords = "Bidentate, Duplex DNA, Self-assembly",
author = "Tagore, {Debarati M.} and Sprinz, {K. Ingrid} and Andrew Hamilton",
year = "2007",
month = "1",
doi = "10.1080/10610270600990253",
language = "English (US)",
volume = "19",
pages = "129--136",
journal = "Supramolecular Chemistry",
issn = "1061-0278",
publisher = "Taylor and Francis Ltd.",
number = "1-2",

}

TY - JOUR

T1 - Duplex DNA as a self-assembling template for the identification of bidentate protein-binding agents

AU - Tagore, Debarati M.

AU - Sprinz, K. Ingrid

AU - Hamilton, Andrew

PY - 2007/1

Y1 - 2007/1

N2 - This paper reviews the versatile self-assembly of bidentate protein-binding agents on a duplex DNA scaffold, as demonstrated using the biotin-streptavidin system. The preferential binding and stabilization of bidentate biotin-containing duplexes is reflected in a 12C increase in the melting temperature of the duplex in the presence of streptavidin.

AB - This paper reviews the versatile self-assembly of bidentate protein-binding agents on a duplex DNA scaffold, as demonstrated using the biotin-streptavidin system. The preferential binding and stabilization of bidentate biotin-containing duplexes is reflected in a 12C increase in the melting temperature of the duplex in the presence of streptavidin.

KW - Bidentate

KW - Duplex DNA

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=34047222690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047222690&partnerID=8YFLogxK

U2 - 10.1080/10610270600990253

DO - 10.1080/10610270600990253

M3 - Article

AN - SCOPUS:34047222690

VL - 19

SP - 129

EP - 136

JO - Supramolecular Chemistry

JF - Supramolecular Chemistry

SN - 1061-0278

IS - 1-2

ER -