Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms

David Dahdal, David C. Reeves, Marc Ruben, Myles H. Akabas, Justin Blau

Research output: Contribution to journalArticle

Abstract

Intercellular signaling is important for accurate circadian rhythms. In Drosophila, the small ventral lateral neurons (s-LNvs) are the dominant pacemaker neurons and set the pace of most other clock neurons in constant darkness. Here we show that two distinct G protein signaling pathways are required in LNvs for 24 hr rhythms. Reducing signaling in LNvs via the G alpha subunit Gs, which signals via cAMP, or via the G alpha subunit Go, which we show signals via Phospholipase 21c, lengthens the period of behavioral rhythms. In contrast, constitutive Gs or Go signaling makes most flies arrhythmic. Using dissociated LNvs in culture, we found that Go and the metabotropic GABAB-R3 receptor are required for the inhibitory effects of GABA on LNvs and that reduced GABAB-R3 expression in vivo lengthens period. Although no clock neurons produce GABA, hyperexciting GABAergic neurons disrupts behavioral rhythms and s-LNv molecular clocks. Therefore, s-LNvs require GABAergic inputs for 24 hr rhythms.

Original languageEnglish (US)
Pages (from-to)964-977
Number of pages14
JournalNeuron
Volume68
Issue number5
DOIs
StatePublished - Dec 9 2010

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Circadian Rhythm
GTP-Binding Proteins
Drosophila
GABAergic Neurons
Neurons
Gs GTP-Binding Protein alpha Subunits
Gi-Go GTP-Binding Protein alpha Subunits
Phospholipases
Darkness
Diptera
gamma-Aminobutyric Acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms. / Dahdal, David; Reeves, David C.; Ruben, Marc; Akabas, Myles H.; Blau, Justin.

In: Neuron, Vol. 68, No. 5, 09.12.2010, p. 964-977.

Research output: Contribution to journalArticle

Dahdal, David ; Reeves, David C. ; Ruben, Marc ; Akabas, Myles H. ; Blau, Justin. / Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms. In: Neuron. 2010 ; Vol. 68, No. 5. pp. 964-977.
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