Draft genome of the filarial nematode parasite Brugia malayi

Elodie Ghedin, Shiliang Wang, David Spiro, Elisabet Caler, Qi Zhao, Jonathan Crabtree, Jonathan E. Allen, Arthur L. Delcher, David B. Guiliano, Diego Miranda-Saavedra, Samuel V. Angiuoli, Todd Creasy, Paolo Amedeo, Brian Haas, Najib M. El-Sayed, Jennifer R. Wortman, Tamara Feldblyum, Luke Tallon, Michael Schatz, Martin ShumwayHean Koo, Steven L. Salzberg, Seth Schobel, Mihaela Pertea, Mihai Pop, Owen White, Geoffrey J. Barton, Clotilde K S Carlow, Michael J. Crawford, Jennifer Daub, Matthew W. Dimmic, Chris F. Estes, Jeremy M. Foster, Mehul Ganatra, William F. Gregory, Nicholas M. Johnson, Ming Jin, Richard Komuniecki, Ian Korf, Sanjay Kumar, Sandra Laney, Ben Wen Li, Wen Li, Tim H. Lindblom, Sara Lustigman, Dong Ma, Claude V. Maina, David M A Martin, James P. McCarter, Larry McReynolds, Makedonka Mitreva, Thomas B. Nutman, John Parkinson, José M. Peregrín-Alvarez, Catherine Poole, Qinghu Ren, Lori Saunders, Ann E. Sluder, Katherine Smith, Mario Stanke, Thomas R. Unnasch, Jenna Ware, Aguan D. Wei, Gary Weil, Deryck J. Williams, Yinhua Zhang, Steven A. Williams, Claire Fraser-Liggett, Barton Slatko, Mark L. Blaxter, Alan L. Scott

Research output: Contribution to journalArticle

Abstract

Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the ∼90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict ∼11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during ∼350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

Original languageEnglish (US)
Pages (from-to)1756-1760
Number of pages5
JournalScience
Volume317
Issue number5845
DOIs
StatePublished - Sep 21 2007

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Brugia malayi
Parasites
Ocular Onchocerciasis
Elephantiasis
Genome
Wolbachia
Synteny
Drug Design
Caenorhabditis elegans
Human Genome
Proteome
Operon
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Ghedin, E., Wang, S., Spiro, D., Caler, E., Zhao, Q., Crabtree, J., ... Scott, A. L. (2007). Draft genome of the filarial nematode parasite Brugia malayi. Science, 317(5845), 1756-1760. https://doi.org/10.1126/science.1145406

Draft genome of the filarial nematode parasite Brugia malayi. / Ghedin, Elodie; Wang, Shiliang; Spiro, David; Caler, Elisabet; Zhao, Qi; Crabtree, Jonathan; Allen, Jonathan E.; Delcher, Arthur L.; Guiliano, David B.; Miranda-Saavedra, Diego; Angiuoli, Samuel V.; Creasy, Todd; Amedeo, Paolo; Haas, Brian; El-Sayed, Najib M.; Wortman, Jennifer R.; Feldblyum, Tamara; Tallon, Luke; Schatz, Michael; Shumway, Martin; Koo, Hean; Salzberg, Steven L.; Schobel, Seth; Pertea, Mihaela; Pop, Mihai; White, Owen; Barton, Geoffrey J.; Carlow, Clotilde K S; Crawford, Michael J.; Daub, Jennifer; Dimmic, Matthew W.; Estes, Chris F.; Foster, Jeremy M.; Ganatra, Mehul; Gregory, William F.; Johnson, Nicholas M.; Jin, Ming; Komuniecki, Richard; Korf, Ian; Kumar, Sanjay; Laney, Sandra; Li, Ben Wen; Li, Wen; Lindblom, Tim H.; Lustigman, Sara; Ma, Dong; Maina, Claude V.; Martin, David M A; McCarter, James P.; McReynolds, Larry; Mitreva, Makedonka; Nutman, Thomas B.; Parkinson, John; Peregrín-Alvarez, José M.; Poole, Catherine; Ren, Qinghu; Saunders, Lori; Sluder, Ann E.; Smith, Katherine; Stanke, Mario; Unnasch, Thomas R.; Ware, Jenna; Wei, Aguan D.; Weil, Gary; Williams, Deryck J.; Zhang, Yinhua; Williams, Steven A.; Fraser-Liggett, Claire; Slatko, Barton; Blaxter, Mark L.; Scott, Alan L.

In: Science, Vol. 317, No. 5845, 21.09.2007, p. 1756-1760.

Research output: Contribution to journalArticle

Ghedin, E, Wang, S, Spiro, D, Caler, E, Zhao, Q, Crabtree, J, Allen, JE, Delcher, AL, Guiliano, DB, Miranda-Saavedra, D, Angiuoli, SV, Creasy, T, Amedeo, P, Haas, B, El-Sayed, NM, Wortman, JR, Feldblyum, T, Tallon, L, Schatz, M, Shumway, M, Koo, H, Salzberg, SL, Schobel, S, Pertea, M, Pop, M, White, O, Barton, GJ, Carlow, CKS, Crawford, MJ, Daub, J, Dimmic, MW, Estes, CF, Foster, JM, Ganatra, M, Gregory, WF, Johnson, NM, Jin, M, Komuniecki, R, Korf, I, Kumar, S, Laney, S, Li, BW, Li, W, Lindblom, TH, Lustigman, S, Ma, D, Maina, CV, Martin, DMA, McCarter, JP, McReynolds, L, Mitreva, M, Nutman, TB, Parkinson, J, Peregrín-Alvarez, JM, Poole, C, Ren, Q, Saunders, L, Sluder, AE, Smith, K, Stanke, M, Unnasch, TR, Ware, J, Wei, AD, Weil, G, Williams, DJ, Zhang, Y, Williams, SA, Fraser-Liggett, C, Slatko, B, Blaxter, ML & Scott, AL 2007, 'Draft genome of the filarial nematode parasite Brugia malayi', Science, vol. 317, no. 5845, pp. 1756-1760. https://doi.org/10.1126/science.1145406
Ghedin E, Wang S, Spiro D, Caler E, Zhao Q, Crabtree J et al. Draft genome of the filarial nematode parasite Brugia malayi. Science. 2007 Sep 21;317(5845):1756-1760. https://doi.org/10.1126/science.1145406
Ghedin, Elodie ; Wang, Shiliang ; Spiro, David ; Caler, Elisabet ; Zhao, Qi ; Crabtree, Jonathan ; Allen, Jonathan E. ; Delcher, Arthur L. ; Guiliano, David B. ; Miranda-Saavedra, Diego ; Angiuoli, Samuel V. ; Creasy, Todd ; Amedeo, Paolo ; Haas, Brian ; El-Sayed, Najib M. ; Wortman, Jennifer R. ; Feldblyum, Tamara ; Tallon, Luke ; Schatz, Michael ; Shumway, Martin ; Koo, Hean ; Salzberg, Steven L. ; Schobel, Seth ; Pertea, Mihaela ; Pop, Mihai ; White, Owen ; Barton, Geoffrey J. ; Carlow, Clotilde K S ; Crawford, Michael J. ; Daub, Jennifer ; Dimmic, Matthew W. ; Estes, Chris F. ; Foster, Jeremy M. ; Ganatra, Mehul ; Gregory, William F. ; Johnson, Nicholas M. ; Jin, Ming ; Komuniecki, Richard ; Korf, Ian ; Kumar, Sanjay ; Laney, Sandra ; Li, Ben Wen ; Li, Wen ; Lindblom, Tim H. ; Lustigman, Sara ; Ma, Dong ; Maina, Claude V. ; Martin, David M A ; McCarter, James P. ; McReynolds, Larry ; Mitreva, Makedonka ; Nutman, Thomas B. ; Parkinson, John ; Peregrín-Alvarez, José M. ; Poole, Catherine ; Ren, Qinghu ; Saunders, Lori ; Sluder, Ann E. ; Smith, Katherine ; Stanke, Mario ; Unnasch, Thomas R. ; Ware, Jenna ; Wei, Aguan D. ; Weil, Gary ; Williams, Deryck J. ; Zhang, Yinhua ; Williams, Steven A. ; Fraser-Liggett, Claire ; Slatko, Barton ; Blaxter, Mark L. ; Scott, Alan L. / Draft genome of the filarial nematode parasite Brugia malayi. In: Science. 2007 ; Vol. 317, No. 5845. pp. 1756-1760.
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abstract = "Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the ∼90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict ∼11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during ∼350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.",
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T1 - Draft genome of the filarial nematode parasite Brugia malayi

AU - Ghedin, Elodie

AU - Wang, Shiliang

AU - Spiro, David

AU - Caler, Elisabet

AU - Zhao, Qi

AU - Crabtree, Jonathan

AU - Allen, Jonathan E.

AU - Delcher, Arthur L.

AU - Guiliano, David B.

AU - Miranda-Saavedra, Diego

AU - Angiuoli, Samuel V.

AU - Creasy, Todd

AU - Amedeo, Paolo

AU - Haas, Brian

AU - El-Sayed, Najib M.

AU - Wortman, Jennifer R.

AU - Feldblyum, Tamara

AU - Tallon, Luke

AU - Schatz, Michael

AU - Shumway, Martin

AU - Koo, Hean

AU - Salzberg, Steven L.

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AU - Barton, Geoffrey J.

AU - Carlow, Clotilde K S

AU - Crawford, Michael J.

AU - Daub, Jennifer

AU - Dimmic, Matthew W.

AU - Estes, Chris F.

AU - Foster, Jeremy M.

AU - Ganatra, Mehul

AU - Gregory, William F.

AU - Johnson, Nicholas M.

AU - Jin, Ming

AU - Komuniecki, Richard

AU - Korf, Ian

AU - Kumar, Sanjay

AU - Laney, Sandra

AU - Li, Ben Wen

AU - Li, Wen

AU - Lindblom, Tim H.

AU - Lustigman, Sara

AU - Ma, Dong

AU - Maina, Claude V.

AU - Martin, David M A

AU - McCarter, James P.

AU - McReynolds, Larry

AU - Mitreva, Makedonka

AU - Nutman, Thomas B.

AU - Parkinson, John

AU - Peregrín-Alvarez, José M.

AU - Poole, Catherine

AU - Ren, Qinghu

AU - Saunders, Lori

AU - Sluder, Ann E.

AU - Smith, Katherine

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AU - Wei, Aguan D.

AU - Weil, Gary

AU - Williams, Deryck J.

AU - Zhang, Yinhua

AU - Williams, Steven A.

AU - Fraser-Liggett, Claire

AU - Slatko, Barton

AU - Blaxter, Mark L.

AU - Scott, Alan L.

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