DNA synthesis and the mitotic cycle in frog kidney cells cultivated in vitro

Research output: Contribution to journalArticle

Abstract

Frog kidney cells in tissue culture exhibit a lag period of about 48 hr before the onset of DNA synthesis. During this time the number of cells incorporating nucleoside into rapidly labeled RNA decreases for 10-12 hr, then increases until 48 hr. Coincident with the start of DNA synthesis there is a sharp drop in the synthesis of rapidly labeled RNA. The generation cycle of frog kidney cells was determined: G1 + telophase = 16 hr, S = 22.3 hr, G2 + prophase = 7.7 hr, T = 46 hr.

Original languageEnglish (US)
Pages (from-to)277-280
Number of pages4
JournalExperimental Cell Research
Volume45
Issue number2
StatePublished - Feb 1967

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Anura
RNA
Telophase
Kidney
Prophase
DNA
Nucleosides
Cell Count
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

DNA synthesis and the mitotic cycle in frog kidney cells cultivated in vitro. / Malamud, Daniel.

In: Experimental Cell Research, Vol. 45, No. 2, 02.1967, p. 277-280.

Research output: Contribution to journalArticle

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