DNA microarray-based mutation discovery and genotyping.

Research output: Contribution to journalArticle

Abstract

DNA microarrays provide an efficient means of identifying single-nucleotide polymorphisms (SNPs) in DNA samples and characterizing their frequencies in individual and mixed samples. We have studied the parameters that determine the sensitivity of DNA probes to SNPs and found that the melting temperature (T (m)) of the probe is the primary determinant of probe sensitivity. An isothermal-melting temperature DNA microarray design, in which the T (m) of all probes is tightly distributed, can be implemented by varying the length of DNA probes within a single DNA microarray. I describe guidelines for designing isothermal-melting temperature DNA microarrays and protocols for labeling and hybridizing DNA samples to DNA microarrays for SNP discovery, genotyping, and quantitative determination of allele frequencies in mixed samples.

Original languageEnglish (US)
Pages (from-to)179-191
Number of pages13
JournalMethods in Molecular Biology
Volume772
StatePublished - 2011

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Oligonucleotide Array Sequence Analysis
Mutation
Freezing
Single Nucleotide Polymorphism
Temperature
DNA Probes
DNA
Gene Frequency
Guidelines

ASJC Scopus subject areas

  • Medicine(all)

Cite this

DNA microarray-based mutation discovery and genotyping. / Gresham, David.

In: Methods in Molecular Biology, Vol. 772, 2011, p. 179-191.

Research output: Contribution to journalArticle

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