DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis

A. A. Alcivar, J. M. Trasler, L. E. Hake, Kourosh Salehi-Ashtiani, E. Goldberg, N. B. Hecht

    Research output: Contribution to journalArticle

    Abstract

    The testis chromatin undergoes profound structural alterations and functional changes during spermatogenesis. Changes in DNA methylation have been correlated with gene expression in a number of systems, but the relationship between methylation and gene expression for testicular genes is unclear. To address this question, DNA methylation patterns and mRNA expression for a somatic form of lactate dehydrogenase (LDH), LDH-A, were compared with those of the testis-specific form, LDH-C, in preparations from testes of prepubertal and sexually mature mice, from isolated testicular cells, and from somatic tissues. At specific sites, LDH-A was less methylated in adult testis than in spleen DNA; the decreased methylation in the testicular DNA occurred as early as type A spermatogonia. In contrast, DNA methylation patterns for LDH-C did not differ between spleen and testis DNAs. In Northern blots, the levels of LDH-A transcripts were low in total testis RNA obtained from 6-12-day-old mice, and in type A and B spermatogonia from 8-day-old mice. LDH-A mRNA levels increased gradually in testes from 16-45-day-old mice. LDH-C transcripts were first detectable in the testes of 12-day-old mice and increased as spermatogenesis proceeded. Both LDH-A and LDH-C mRNA levels were low in preleptotene spermatocytes and leptotenetes and round spermatids. Reduced levels of LDH-A and LDH-C mRNAs were found in the residual bodies/cytoplasts fraction. Analysis of polysomal gladients from total testis indicated that although both LDH-A and LDHC mRNAs are translationally regulated, a greater proportion of the LDH-C mRNA was present in polysomes. In summary, our results indicate that whereas DNA methylation of LDH-A and LDH-C at the 5'-CCGG-3' sites monitored here do not change markedly during testis development, both genes are temporally expressed.

    Original languageEnglish (US)
    Pages (from-to)527-535
    Number of pages9
    JournalBiology of Reproduction
    Volume44
    Issue number3
    DOIs
    StatePublished - Jan 1 1991

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    DNA Methylation
    Spermatogenesis
    Testis
    Rodentia
    Gene Expression
    Messenger RNA
    Spermatogonia
    Spleen
    lactate dehydrogenase 5
    lactate dehydrogenase C4
    Spermatocytes
    Polyribosomes
    Spermatids
    DNA
    L-Lactate Dehydrogenase
    Northern Blotting
    Methylation
    Genes
    Chromatin
    RNA

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Alcivar, A. A., Trasler, J. M., Hake, L. E., Salehi-Ashtiani, K., Goldberg, E., & Hecht, N. B. (1991). DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis. Biology of Reproduction, 44(3), 527-535. https://doi.org/10.1095/biolreprod44.3.527

    DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis. / Alcivar, A. A.; Trasler, J. M.; Hake, L. E.; Salehi-Ashtiani, Kourosh; Goldberg, E.; Hecht, N. B.

    In: Biology of Reproduction, Vol. 44, No. 3, 01.01.1991, p. 527-535.

    Research output: Contribution to journalArticle

    Alcivar, AA, Trasler, JM, Hake, LE, Salehi-Ashtiani, K, Goldberg, E & Hecht, NB 1991, 'DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis', Biology of Reproduction, vol. 44, no. 3, pp. 527-535. https://doi.org/10.1095/biolreprod44.3.527
    Alcivar, A. A. ; Trasler, J. M. ; Hake, L. E. ; Salehi-Ashtiani, Kourosh ; Goldberg, E. ; Hecht, N. B. / DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis. In: Biology of Reproduction. 1991 ; Vol. 44, No. 3. pp. 527-535.
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    abstract = "The testis chromatin undergoes profound structural alterations and functional changes during spermatogenesis. Changes in DNA methylation have been correlated with gene expression in a number of systems, but the relationship between methylation and gene expression for testicular genes is unclear. To address this question, DNA methylation patterns and mRNA expression for a somatic form of lactate dehydrogenase (LDH), LDH-A, were compared with those of the testis-specific form, LDH-C, in preparations from testes of prepubertal and sexually mature mice, from isolated testicular cells, and from somatic tissues. At specific sites, LDH-A was less methylated in adult testis than in spleen DNA; the decreased methylation in the testicular DNA occurred as early as type A spermatogonia. In contrast, DNA methylation patterns for LDH-C did not differ between spleen and testis DNAs. In Northern blots, the levels of LDH-A transcripts were low in total testis RNA obtained from 6-12-day-old mice, and in type A and B spermatogonia from 8-day-old mice. LDH-A mRNA levels increased gradually in testes from 16-45-day-old mice. LDH-C transcripts were first detectable in the testes of 12-day-old mice and increased as spermatogenesis proceeded. Both LDH-A and LDH-C mRNA levels were low in preleptotene spermatocytes and leptotenetes and round spermatids. Reduced levels of LDH-A and LDH-C mRNAs were found in the residual bodies/cytoplasts fraction. Analysis of polysomal gladients from total testis indicated that although both LDH-A and LDHC mRNAs are translationally regulated, a greater proportion of the LDH-C mRNA was present in polysomes. In summary, our results indicate that whereas DNA methylation of LDH-A and LDH-C at the 5'-CCGG-3' sites monitored here do not change markedly during testis development, both genes are temporally expressed.",
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