Dlx genes pattern mammalian jaw primordium by regulating both lower jaw-specific and upper jaw-specific genetic programs

Juhee Jeong, Xue Li, Robert J. McEvilly, Michael G. Rosenfeld, Thomas Lufkin, John L R Rubenstein

Research output: Contribution to journalArticle

Abstract

Dlx transcription factors are implicated in patterning the mammalian jaw, base on their nested expression patterns in the first branchial arch (primordiurn for jaw) and mutant phenotypes; inactivation of Dlx1 and Dlx2 (Dlx1/2-l-) causes defects in the upper jaw, whereas Dlx5/6-l- results in homeotic transformation of the lower jaw into upper jaw. Therefore, the'Dlx codes' appear to regionalize the jaw primordium such that Dlx1/2 regulate upper jaw development, while Dlx5/6 confer the lower jaw fate. Towards identifying the genetic pathways downstream of Dlx5/6, we compared the gene expression profiles of the wild-type and Dlx5/6-l- mouse mandibular arch (prospective lower jaw). We identified 20 previously unrecognized Dlx5/ 6-downstream genes, of which 12 were downregulated and 8 upregulated in the mutant. We found a Dlx-regulated transcriptional enhancer in close proximity to Gbx2, one of the Dlx5/6-downstream genes, strongly suggesting that Gbx2 is a direct target of Dlx5/6. We also showed that Pou3f3 is normally expressed in the maxillary (prospective upper jaw) but not mandibular arch, is upregulated in the mandibular arch of Dlx5/6-l-, and is essential for formation of some of the maxillary arch-derived skeleton. A comparative analysis of the morphological and molecular phenotypes of various Dlx single and double mutants revealed that Dlx1, 2, 5 and 6 act both partially redundantly and antagonistically to direct differential expression of downstream genes in each domain of the first branchial arch. We propose a new model for Dlx-mediated mammalian jaw patterning.

Original languageEnglish (US)
Pages (from-to)2905-2916
Number of pages12
JournalDevelopment
Volume135
Issue number17
DOIs
StatePublished - Sep 2008

Fingerprint

Maxilla
Jaw
Genes
Branchial Region
Phenotype
Transcriptome
Skeleton
Transcription Factors
Down-Regulation
Gene Expression

Keywords

  • Branchial arch
  • Craniofacial
  • Dlx
  • Gbx2
  • Jaw
  • Mouse
  • Pou3f3

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Dlx genes pattern mammalian jaw primordium by regulating both lower jaw-specific and upper jaw-specific genetic programs. / Jeong, Juhee; Li, Xue; McEvilly, Robert J.; Rosenfeld, Michael G.; Lufkin, Thomas; Rubenstein, John L R.

In: Development, Vol. 135, No. 17, 09.2008, p. 2905-2916.

Research output: Contribution to journalArticle

Jeong, Juhee ; Li, Xue ; McEvilly, Robert J. ; Rosenfeld, Michael G. ; Lufkin, Thomas ; Rubenstein, John L R. / Dlx genes pattern mammalian jaw primordium by regulating both lower jaw-specific and upper jaw-specific genetic programs. In: Development. 2008 ; Vol. 135, No. 17. pp. 2905-2916.
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