Dkk2 promotes neural crest specification by activating Wnt/β-catenin signaling in a GSK3β independent manner

Arun Devotta, Chang Soo Hong, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

Neural crest progenitors are specified through the modulation of several signaling pathways, among which the activation of Wnt/β-catenin signaling by Wnt8 is especially critical. Glycoproteins of the Dickkopf (Dkk) family are important modulators of Wnt signaling acting primarily as Wnt antagonists. Here we report that Dkk2 is required for neural crest specification functioning as a positive regulator of Wnt/β-catenin signaling. Dkk2 depletion in Xenopus embryos causes a loss of neural crest progenitors, a phenotype that is rescued by expression of Lrp6 or β-catenin. Dkk2 overexpression expands the neural crest territory in a pattern reminiscent of Wnt8, Lrp6 and β-catenin gain-of-function phenotypes. Mechanistically, we show that Dkk2 mediates its neural crest-inducing activity through Lrp6 and β-catenin, however unlike Wnt8, in a GSK3b independent manner. These findings suggest that Wnt8 and Dkk2 converge on β-catenin using distinct transduction pathways both independently required to activate Wnt/β-catenin signaling and induce neural crest cells.

Original languageEnglish (US)
Article numbere34404
JournaleLife
Volume7
DOIs
StatePublished - Jul 23 2018

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Catenins
Neural Crest
Specifications
Phenotype
Xenopus
Modulators
Glycoproteins
Embryonic Structures
Chemical activation
Modulation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Dkk2 promotes neural crest specification by activating Wnt/β-catenin signaling in a GSK3β independent manner. / Devotta, Arun; Hong, Chang Soo; Saint-Jeannet, Jean-Pierre.

In: eLife, Vol. 7, e34404, 23.07.2018.

Research output: Contribution to journalArticle

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