Diversification in wild populations of the model organism Anolis carolinensis: A genome-wide phylogeographic investigation

Joseph D. Manthey, Marc Tollis, Alan R. Lemmon, Emily Moriarty Lemmon, Stephane Boissinot

Research output: Contribution to journalArticle

Abstract

The green anole (Anolis carolinensis) is a lizard widespread throughout the southeastern United States and is a model organism for the study of reproductive behavior, physiology, neural biology, and genomics. Previous phylogeographic studies of A.�carolinensis using mitochondrial DNA and small numbers of nuclear loci identified conflicting and poorly supported relationships among geographically structured clades; these inconsistencies preclude confident use of A.�carolinensis evolutionary history in association with morphological, physiological, or reproductive biology studies among sampling localities and necessitate increased effort to resolve evolutionary relationships among natural populations. Here, we used anchored hybrid enrichment of hundreds of genetic markers across the genome of A.�carolinensis and identified five strongly supported phylogeographic groups. Using multiple analyses, we produced a fully resolved species tree, investigated relative support for each lineage across all gene trees, and identified mito-nuclear discordance when comparing our results to previous studies. We found fixed differences in only one clade—southern Florida restricted to the Everglades region—while most polymorphisms were shared between lineages. The southern Florida group likely diverged from other populations during the Pliocene, with all other diversification during the Pleistocene. Multiple lines of support, including phylogenetic relationships, a latitudinal gradient in genetic diversity, and relatively more stable long-term population sizes in southern phylogeographic groups, indicate that diversification in A.�carolinensis occurred northward from southern Florida.

Original languageEnglish (US)
Pages (from-to)8115-8125
Number of pages11
JournalEcology and Evolution
Volume6
Issue number22
DOIs
StatePublished - Nov 1 2016

Fingerprint

Anolis carolinensis
wild population
genome
reproductive behavior
latitudinal gradient
organisms
genetic marker
reproductive biology
lizard
mitochondrial DNA
physiology
population size
Biological Sciences
genomics
Pliocene
polymorphism
Pleistocene
Southeastern United States
phylogenetics
lizards

Keywords

  • Anolis
  • phylogeography
  • species tree
  • target capture

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Cite this

Diversification in wild populations of the model organism Anolis carolinensis : A genome-wide phylogeographic investigation. / Manthey, Joseph D.; Tollis, Marc; Lemmon, Alan R.; Moriarty Lemmon, Emily; Boissinot, Stephane.

In: Ecology and Evolution, Vol. 6, No. 22, 01.11.2016, p. 8115-8125.

Research output: Contribution to journalArticle

Manthey, Joseph D. ; Tollis, Marc ; Lemmon, Alan R. ; Moriarty Lemmon, Emily ; Boissinot, Stephane. / Diversification in wild populations of the model organism Anolis carolinensis : A genome-wide phylogeographic investigation. In: Ecology and Evolution. 2016 ; Vol. 6, No. 22. pp. 8115-8125.
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