Diversification in wild populations of the model organism Anolis carolinensis

A genome-wide phylogeographic investigation

Joseph D. Manthey, Marc Tollis, Alan R. Lemmon, Emily Moriarty Lemmon, Stephane Boissinot

    Research output: Contribution to journalArticle

    Abstract

    The green anole (Anolis carolinensis) is a lizard widespread throughout the southeastern United States and is a model organism for the study of reproductive behavior, physiology, neural biology, and genomics. Previous phylogeographic studies of A.�carolinensis using mitochondrial DNA and small numbers of nuclear loci identified conflicting and poorly supported relationships among geographically structured clades; these inconsistencies preclude confident use of A.�carolinensis evolutionary history in association with morphological, physiological, or reproductive biology studies among sampling localities and necessitate increased effort to resolve evolutionary relationships among natural populations. Here, we used anchored hybrid enrichment of hundreds of genetic markers across the genome of A.�carolinensis and identified five strongly supported phylogeographic groups. Using multiple analyses, we produced a fully resolved species tree, investigated relative support for each lineage across all gene trees, and identified mito-nuclear discordance when comparing our results to previous studies. We found fixed differences in only one clade—southern Florida restricted to the Everglades region—while most polymorphisms were shared between lineages. The southern Florida group likely diverged from other populations during the Pliocene, with all other diversification during the Pleistocene. Multiple lines of support, including phylogenetic relationships, a latitudinal gradient in genetic diversity, and relatively more stable long-term population sizes in southern phylogeographic groups, indicate that diversification in A.�carolinensis occurred northward from southern Florida.

    Original languageEnglish (US)
    Pages (from-to)8115-8125
    Number of pages11
    JournalEcology and Evolution
    Volume6
    Issue number22
    DOIs
    StatePublished - Nov 1 2016

    Fingerprint

    Anolis carolinensis
    wild population
    genome
    reproductive behavior
    latitudinal gradient
    organisms
    genetic marker
    reproductive biology
    lizard
    mitochondrial DNA
    physiology
    population size
    Biological Sciences
    genomics
    Pliocene
    polymorphism
    Pleistocene
    Southeastern United States
    phylogenetics
    lizards

    Keywords

    • Anolis
    • phylogeography
    • species tree
    • target capture

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Ecology
    • Nature and Landscape Conservation

    Cite this

    Diversification in wild populations of the model organism Anolis carolinensis : A genome-wide phylogeographic investigation. / Manthey, Joseph D.; Tollis, Marc; Lemmon, Alan R.; Moriarty Lemmon, Emily; Boissinot, Stephane.

    In: Ecology and Evolution, Vol. 6, No. 22, 01.11.2016, p. 8115-8125.

    Research output: Contribution to journalArticle

    Manthey, Joseph D. ; Tollis, Marc ; Lemmon, Alan R. ; Moriarty Lemmon, Emily ; Boissinot, Stephane. / Diversification in wild populations of the model organism Anolis carolinensis : A genome-wide phylogeographic investigation. In: Ecology and Evolution. 2016 ; Vol. 6, No. 22. pp. 8115-8125.
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