Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience

Silvia Argimón, Page W. Caufield

Research output: Contribution to journalArticle

Abstract

Streptococcus mutans, a member of the human oral flora, is a widely recognized etiological agent of dental caries. The cariogenic potential of S. mutans is related to its ability to metabolize a wide variety of sugars, form a robust biofilm, produce copious amounts of lactic acid, and thrive in the acid environment that it generates. The remarkable genetic variability present within the species is reflected at the phenotypic level, notably in the differences in the cariogenic potential between strains. However, the genetic basis of these differences is yet to be elucidated. In this study, we surveyed by PCR and DNA hybridization the distribution of putative virulence genes, genomic islands, and insertion sequences across a collection of 33 strains isolated from either children with severe early childhood caries (S-ECC) or those who were caries free (CF). We found this genetically diverse group of isolates to be remarkably homogeneous with regard to the distribution of the putative virulence genes and genetic elements analyzed. Our findings point to the role of other factors in the pathogenesis of S-ECC, such as uncharacterized virulence genes, differences in gene expression and/or enzymatic activity, cooperation between S. mutans strains or with other members of the oral biota, and host factors.

Original languageEnglish (US)
Pages (from-to)984-992
Number of pages9
JournalJournal of Clinical Microbiology
Volume49
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Streptococcus mutans
Virulence
Genes
Genomic Islands
Biota
Insertional Mutagenesis
Dental Caries
Biofilms
Lactic Acid
Gene Expression
Polymerase Chain Reaction
Acids
DNA

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience. / Argimón, Silvia; Caufield, Page W.

In: Journal of Clinical Microbiology, Vol. 49, No. 3, 03.2011, p. 984-992.

Research output: Contribution to journalArticle

@article{ca448705108742de96d9494997447583,
title = "Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience",
abstract = "Streptococcus mutans, a member of the human oral flora, is a widely recognized etiological agent of dental caries. The cariogenic potential of S. mutans is related to its ability to metabolize a wide variety of sugars, form a robust biofilm, produce copious amounts of lactic acid, and thrive in the acid environment that it generates. The remarkable genetic variability present within the species is reflected at the phenotypic level, notably in the differences in the cariogenic potential between strains. However, the genetic basis of these differences is yet to be elucidated. In this study, we surveyed by PCR and DNA hybridization the distribution of putative virulence genes, genomic islands, and insertion sequences across a collection of 33 strains isolated from either children with severe early childhood caries (S-ECC) or those who were caries free (CF). We found this genetically diverse group of isolates to be remarkably homogeneous with regard to the distribution of the putative virulence genes and genetic elements analyzed. Our findings point to the role of other factors in the pathogenesis of S-ECC, such as uncharacterized virulence genes, differences in gene expression and/or enzymatic activity, cooperation between S. mutans strains or with other members of the oral biota, and host factors.",
author = "Silvia Argim{\'o}n and Caufield, {Page W.}",
year = "2011",
month = "3",
doi = "10.1128/JCM.01993-10",
language = "English (US)",
volume = "49",
pages = "984--992",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience

AU - Argimón, Silvia

AU - Caufield, Page W.

PY - 2011/3

Y1 - 2011/3

N2 - Streptococcus mutans, a member of the human oral flora, is a widely recognized etiological agent of dental caries. The cariogenic potential of S. mutans is related to its ability to metabolize a wide variety of sugars, form a robust biofilm, produce copious amounts of lactic acid, and thrive in the acid environment that it generates. The remarkable genetic variability present within the species is reflected at the phenotypic level, notably in the differences in the cariogenic potential between strains. However, the genetic basis of these differences is yet to be elucidated. In this study, we surveyed by PCR and DNA hybridization the distribution of putative virulence genes, genomic islands, and insertion sequences across a collection of 33 strains isolated from either children with severe early childhood caries (S-ECC) or those who were caries free (CF). We found this genetically diverse group of isolates to be remarkably homogeneous with regard to the distribution of the putative virulence genes and genetic elements analyzed. Our findings point to the role of other factors in the pathogenesis of S-ECC, such as uncharacterized virulence genes, differences in gene expression and/or enzymatic activity, cooperation between S. mutans strains or with other members of the oral biota, and host factors.

AB - Streptococcus mutans, a member of the human oral flora, is a widely recognized etiological agent of dental caries. The cariogenic potential of S. mutans is related to its ability to metabolize a wide variety of sugars, form a robust biofilm, produce copious amounts of lactic acid, and thrive in the acid environment that it generates. The remarkable genetic variability present within the species is reflected at the phenotypic level, notably in the differences in the cariogenic potential between strains. However, the genetic basis of these differences is yet to be elucidated. In this study, we surveyed by PCR and DNA hybridization the distribution of putative virulence genes, genomic islands, and insertion sequences across a collection of 33 strains isolated from either children with severe early childhood caries (S-ECC) or those who were caries free (CF). We found this genetically diverse group of isolates to be remarkably homogeneous with regard to the distribution of the putative virulence genes and genetic elements analyzed. Our findings point to the role of other factors in the pathogenesis of S-ECC, such as uncharacterized virulence genes, differences in gene expression and/or enzymatic activity, cooperation between S. mutans strains or with other members of the oral biota, and host factors.

UR - http://www.scopus.com/inward/record.url?scp=79952335654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952335654&partnerID=8YFLogxK

U2 - 10.1128/JCM.01993-10

DO - 10.1128/JCM.01993-10

M3 - Article

VL - 49

SP - 984

EP - 992

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -