Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry

Yi Sheng Wang, Sahadevan Sabu, Shih Chia Wei, C. M. Josh Kao, Xianglei Kong, Shing Chih Liau, Chau Chung Han, Huan Cheng Chang, Shih Yu Tu, A. H. Kung, John Zhang

Research output: Contribution to journalArticle

Abstract

Detachment of heme prosthetic groups from gaseous myoglobin ions has been studied by collision-induced dissociation and infrared multiphoton dissociation in combination with Fourier-transform ion cyclotron resonance mass spectrometry. Multiply charged holomyoglobin ions (hMbn+) were generated by electrospray ionization and transferred to an ion cyclotron resonance cell, where the ions of interest were isolated and fragmented by either collision with Ar atoms or irradiation with 3 μm photons, producing apomyoglobin ions (aMbn+). Both charged heme loss (with [Fe (III) -heme]+ and aMb (n-1) + as the products) and neutral heme loss (with [Fe(II)-heme] and aMbn+ as the products) were detected concurrently for hMbn+ produced from a myoglobin solution pretreated with reducing reagents. By reference to Ea =0.9 eV determined by blackbody infrared radiative dissociation for charged heme loss of ferric hMbn+, an activation energy of 1.1 eV was deduced for neutral heme loss of ferrous hMbn+ with n=9 and 10.

Original languageEnglish (US)
Article number133310
JournalJournal of Chemical Physics
Volume125
Issue number13
DOIs
StatePublished - 2006

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Cyclotron resonance
myoglobin
Myoglobin
cyclotron resonance
Heme
Mass spectrometry
Fourier transforms
mass spectroscopy
Spectroscopy
dissociation
Ions
Infrared radiation
spectroscopy
ions
collisions
Electrospray ionization
products
detachment
reagents
Prosthetics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry. / Wang, Yi Sheng; Sabu, Sahadevan; Wei, Shih Chia; Josh Kao, C. M.; Kong, Xianglei; Liau, Shing Chih; Han, Chau Chung; Chang, Huan Cheng; Tu, Shih Yu; Kung, A. H.; Zhang, John.

In: Journal of Chemical Physics, Vol. 125, No. 13, 133310, 2006.

Research output: Contribution to journalArticle

Wang, Yi Sheng ; Sabu, Sahadevan ; Wei, Shih Chia ; Josh Kao, C. M. ; Kong, Xianglei ; Liau, Shing Chih ; Han, Chau Chung ; Chang, Huan Cheng ; Tu, Shih Yu ; Kung, A. H. ; Zhang, John. / Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry. In: Journal of Chemical Physics. 2006 ; Vol. 125, No. 13.
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AU - Wang, Yi Sheng

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AU - Wei, Shih Chia

AU - Josh Kao, C. M.

AU - Kong, Xianglei

AU - Liau, Shing Chih

AU - Han, Chau Chung

AU - Chang, Huan Cheng

AU - Tu, Shih Yu

AU - Kung, A. H.

AU - Zhang, John

PY - 2006

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N2 - Detachment of heme prosthetic groups from gaseous myoglobin ions has been studied by collision-induced dissociation and infrared multiphoton dissociation in combination with Fourier-transform ion cyclotron resonance mass spectrometry. Multiply charged holomyoglobin ions (hMbn+) were generated by electrospray ionization and transferred to an ion cyclotron resonance cell, where the ions of interest were isolated and fragmented by either collision with Ar atoms or irradiation with 3 μm photons, producing apomyoglobin ions (aMbn+). Both charged heme loss (with [Fe (III) -heme]+ and aMb (n-1) + as the products) and neutral heme loss (with [Fe(II)-heme] and aMbn+ as the products) were detected concurrently for hMbn+ produced from a myoglobin solution pretreated with reducing reagents. By reference to Ea =0.9 eV determined by blackbody infrared radiative dissociation for charged heme loss of ferric hMbn+, an activation energy of 1.1 eV was deduced for neutral heme loss of ferrous hMbn+ with n=9 and 10.

AB - Detachment of heme prosthetic groups from gaseous myoglobin ions has been studied by collision-induced dissociation and infrared multiphoton dissociation in combination with Fourier-transform ion cyclotron resonance mass spectrometry. Multiply charged holomyoglobin ions (hMbn+) were generated by electrospray ionization and transferred to an ion cyclotron resonance cell, where the ions of interest were isolated and fragmented by either collision with Ar atoms or irradiation with 3 μm photons, producing apomyoglobin ions (aMbn+). Both charged heme loss (with [Fe (III) -heme]+ and aMb (n-1) + as the products) and neutral heme loss (with [Fe(II)-heme] and aMbn+ as the products) were detected concurrently for hMbn+ produced from a myoglobin solution pretreated with reducing reagents. By reference to Ea =0.9 eV determined by blackbody infrared radiative dissociation for charged heme loss of ferric hMbn+, an activation energy of 1.1 eV was deduced for neutral heme loss of ferrous hMbn+ with n=9 and 10.

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