Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains

C. Murre, E. Choi, J. Weis, J. G. Seidman, K. Ozato, L. Liu, S. J. Burakoff, C. S. Reiss

Research output: Contribution to journalArticle

Abstract

A novel H-2 gene in which the first external (N) domain of the H-2L(d) antigen was replaced with that of the H-2D(d) antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2L(d) and H-2D(d) antigens. It was found that serological determinants are distributed both in the N (D(d)) and C1 (L(d)) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and C1 domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.

Original languageEnglish (US)
Pages (from-to)167-178
Number of pages12
JournalJournal of Experimental Medicine
Volume160
Issue number1
StatePublished - 1984

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Histocompatibility Antigen H-2D
Histocompatibility Antigens
T-Lymphocyte Epitopes
Cytotoxic T-Lymphocytes
H-2 Antigens
Dissection
Genes
Viruses
Vesicular Stomatitis
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

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Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains. / Murre, C.; Choi, E.; Weis, J.; Seidman, J. G.; Ozato, K.; Liu, L.; Burakoff, S. J.; Reiss, C. S.

In: Journal of Experimental Medicine, Vol. 160, No. 1, 1984, p. 167-178.

Research output: Contribution to journalArticle

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AU - Weis, J.

AU - Seidman, J. G.

AU - Ozato, K.

AU - Liu, L.

AU - Burakoff, S. J.

AU - Reiss, C. S.

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AB - A novel H-2 gene in which the first external (N) domain of the H-2L(d) antigen was replaced with that of the H-2D(d) antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2L(d) and H-2D(d) antigens. It was found that serological determinants are distributed both in the N (D(d)) and C1 (L(d)) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and C1 domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.

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