Abstract
A novel H-2 gene in which the first external (N) domain of the H-2L(d) antigen was replaced with that of the H-2D(d) antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2L(d) and H-2D(d) antigens. It was found that serological determinants are distributed both in the N (D(d)) and C1 (L(d)) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and C1 domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-178 |
| Number of pages | 12 |
| Journal | Journal of Experimental Medicine |
| Volume | 160 |
| Issue number | 1 |
| State | Published - 1984 |
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ASJC Scopus subject areas
- Immunology
Cite this
Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains. / Murre, C.; Choi, E.; Weis, J.; Seidman, J. G.; Ozato, K.; Liu, L.; Burakoff, S. J.; Reiss, C. S.
In: Journal of Experimental Medicine, Vol. 160, No. 1, 1984, p. 167-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains
AU - Murre, C.
AU - Choi, E.
AU - Weis, J.
AU - Seidman, J. G.
AU - Ozato, K.
AU - Liu, L.
AU - Burakoff, S. J.
AU - Reiss, C. S.
PY - 1984
Y1 - 1984
N2 - A novel H-2 gene in which the first external (N) domain of the H-2L(d) antigen was replaced with that of the H-2D(d) antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2L(d) and H-2D(d) antigens. It was found that serological determinants are distributed both in the N (D(d)) and C1 (L(d)) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and C1 domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.
AB - A novel H-2 gene in which the first external (N) domain of the H-2L(d) antigen was replaced with that of the H-2D(d) antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2L(d) and H-2D(d) antigens. It was found that serological determinants are distributed both in the N (D(d)) and C1 (L(d)) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and C1 domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.
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M3 - Article
VL - 160
SP - 167
EP - 178
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 1
ER -