Disposable polydimethylsiloxane/silicon hybrid chips for protein detection

Shifeng Li, Pierre N. Floriano, Nicolaos Christodoulides, David Y. Fozdar, Dongbing Shao, Mehnaaz F. Ali, Priya Dharshan, Sanghamitra Mohanty, Dean Neikirk, John McDevitt, Shaochen Chen

Research output: Contribution to journalArticle

Abstract

This paper presents disposable protein analysis chips with single- or four-chamber - constructed from poly(dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting polymeric beads. The sample is introduced into the fluid network through the top PDMS layer, where it is directed to the bead chamber. After reaction of the analyte with the probe beads, the signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single-chamber protein chip was found to be 1 ng/ml. Additionally, using a back pressure compensation method, the signals from each chamber of the four-chamber chip were found to fall within 10% of each other.

Original languageEnglish (US)
Pages (from-to)574-580
Number of pages7
JournalBiosensors and Bioelectronics
Volume21
Issue number4
DOIs
StatePublished - Oct 15 2005

Fingerprint

Protein Array Analysis
Silicon
Polydimethylsiloxane
Proteins
Microcavities
CCD cameras
C-Reactive Protein
Limit of Detection
Assays
Multilayers
Pressure
Fluids
baysilon

Keywords

  • C-reactive protein
  • Disposable
  • Poly(dimethylsiloxane)
  • Taste chip

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Li, S., Floriano, P. N., Christodoulides, N., Fozdar, D. Y., Shao, D., Ali, M. F., ... Chen, S. (2005). Disposable polydimethylsiloxane/silicon hybrid chips for protein detection. Biosensors and Bioelectronics, 21(4), 574-580. https://doi.org/10.1016/j.bios.2004.12.010

Disposable polydimethylsiloxane/silicon hybrid chips for protein detection. / Li, Shifeng; Floriano, Pierre N.; Christodoulides, Nicolaos; Fozdar, David Y.; Shao, Dongbing; Ali, Mehnaaz F.; Dharshan, Priya; Mohanty, Sanghamitra; Neikirk, Dean; McDevitt, John; Chen, Shaochen.

In: Biosensors and Bioelectronics, Vol. 21, No. 4, 15.10.2005, p. 574-580.

Research output: Contribution to journalArticle

Li, S, Floriano, PN, Christodoulides, N, Fozdar, DY, Shao, D, Ali, MF, Dharshan, P, Mohanty, S, Neikirk, D, McDevitt, J & Chen, S 2005, 'Disposable polydimethylsiloxane/silicon hybrid chips for protein detection', Biosensors and Bioelectronics, vol. 21, no. 4, pp. 574-580. https://doi.org/10.1016/j.bios.2004.12.010
Li S, Floriano PN, Christodoulides N, Fozdar DY, Shao D, Ali MF et al. Disposable polydimethylsiloxane/silicon hybrid chips for protein detection. Biosensors and Bioelectronics. 2005 Oct 15;21(4):574-580. https://doi.org/10.1016/j.bios.2004.12.010
Li, Shifeng ; Floriano, Pierre N. ; Christodoulides, Nicolaos ; Fozdar, David Y. ; Shao, Dongbing ; Ali, Mehnaaz F. ; Dharshan, Priya ; Mohanty, Sanghamitra ; Neikirk, Dean ; McDevitt, John ; Chen, Shaochen. / Disposable polydimethylsiloxane/silicon hybrid chips for protein detection. In: Biosensors and Bioelectronics. 2005 ; Vol. 21, No. 4. pp. 574-580.
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