Discoidin-domain receptor coordinates cell-matrix adhesion and collective polarity in migratory cardiopharyngeal progenitors

Yelena Y. Bernadskaya, Saahil Brahmbhatt, Stephanie E. Gline, Wei Wang, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

Integrated analyses of regulated effector genes, cellular processes, and extrinsic signals are required to understand how transcriptional networks coordinate fate specification and cell behavior during embryogenesis. Ciona cardiopharyngeal progenitors, the trunk ventral cells (TVCs), polarize as leader and trailer cells that migrate between the ventral epidermis and trunk endoderm. We show that the TVC-specific collagen-binding Discoidin-domain receptor (Ddr) cooperates with Integrin-β1 to promote cell-matrix adhesion. We find that endodermal cells secrete a collagen, Col9-a1, that is deposited in the basal epidermal matrix and promotes Ddr activation at the ventral membrane of migrating TVCs. A functional antagonism between Ddr/Intβ1-mediated cell-matrix adhesion and Vegfr signaling appears to modulate the position of cardiopharyngeal progenitors between the endoderm and epidermis. We show that Ddr promotes leader-trailer-polarized BMP-Smad signaling independently of its role in cell-matrix adhesion. We propose that dual functions of Ddr integrate transcriptional inputs to coordinate subcellular processes underlying collective polarity and migration.

Original languageEnglish (US)
Article number57
JournalNature Communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

Cell-Matrix Junctions
polarity
adhesion
Adhesion
Light trailers
matrices
cells
Endoderm
Collagen
trailers
Epidermis
epidermis
Integrins
collagens
Genes
Gene Regulatory Networks
Chemical activation
Membranes
Specifications
Embryonic Development

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Discoidin-domain receptor coordinates cell-matrix adhesion and collective polarity in migratory cardiopharyngeal progenitors. / Bernadskaya, Yelena Y.; Brahmbhatt, Saahil; Gline, Stephanie E.; Wang, Wei; Christiaen, Lionel.

In: Nature Communications, Vol. 10, No. 1, 57, 01.12.2019.

Research output: Contribution to journalArticle

Bernadskaya, Yelena Y. ; Brahmbhatt, Saahil ; Gline, Stephanie E. ; Wang, Wei ; Christiaen, Lionel. / Discoidin-domain receptor coordinates cell-matrix adhesion and collective polarity in migratory cardiopharyngeal progenitors. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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