Directing neuron-specific transgene expression in the mouse CNS

Goichi Miyoshi, Gordon Fishell

    Research output: Contribution to journalReview article

    Abstract

    Recent advances in molecular genetics have produced many novel strategies for directing the expression of both functional and regulatory elements in transgenic mice. With the application of such approaches, the specific populations that comprise CNS networks can be both visualized and manipulated. Transgenic methods now range from the use of specific enhancer elements and large genomic regions assembled using BACs and PACs, to the use of gene targeting to a specific locus. In addition, the advent of transactivators and site-specific recombinases has provided unprecedented spatial and temporal control for directing expression in the CNS using a combination of appropriate alleles. As a result, the promise of being able to use transgenics to target specific neuronal populations is now being realized.

    Original languageEnglish (US)
    Pages (from-to)577-584
    Number of pages8
    JournalCurrent Opinion in Neurobiology
    Volume16
    Issue number5
    DOIs
    StatePublished - Oct 1 2006

    Fingerprint

    Transgenes
    Neurons
    Trans-Activators
    Gene Targeting
    Transgenic Mice
    Population
    Molecular Biology
    Alleles
    Site-specific recombinase

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Directing neuron-specific transgene expression in the mouse CNS. / Miyoshi, Goichi; Fishell, Gordon.

    In: Current Opinion in Neurobiology, Vol. 16, No. 5, 01.10.2006, p. 577-584.

    Research output: Contribution to journalReview article

    Miyoshi, Goichi ; Fishell, Gordon. / Directing neuron-specific transgene expression in the mouse CNS. In: Current Opinion in Neurobiology. 2006 ; Vol. 16, No. 5. pp. 577-584.
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