Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture

T. Lin, J. Haskell, N. Vinson, Louis Terracio

Research output: Contribution to journalArticle

Abstract

Insulin-like growth factor-I (IGF-I) in concentration as low as 10 ng/ml significantly increased basal testosterone formation and 100 ng/ml of IGF-I increased testosterone production more than two fold in primary cultures of purified mature Leydig cells. IGF-I also markedly potentiated hCG-induced testosterone formation in a dose-dependent manner. Furthermore, IGF-I enhanced 8-bromo cyclic AMP induced steroidogenesis and hCG-stimulated cyclic AMP formation. The binding of 125I-IGF-I to purified Leydig cells was linear with a binding affinity of 0.56 ± 0.07×109 M-1 and a capacity of 167 ± 10.2 fmol/mg protein. Insulin and multiplication-stimulating activity were less potent than IGF-I in competing the binding of 125I-IGF-I to purified Leydig cells. This suggests that Leydig cells contain specific type I IGF receptor and IGF-I could modulate Leydig cell steroidogenesis.

Original languageEnglish (US)
Pages (from-to)950-956
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number3
DOIs
StatePublished - Jun 30 1986

Fingerprint

Leydig Cells
Insulin-Like Growth Factor I
Testosterone
IGF Type 1 Receptor
8-Bromo Cyclic Adenosine Monophosphate
Insulin-Like Growth Factor II
Cyclic AMP
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture. / Lin, T.; Haskell, J.; Vinson, N.; Terracio, Louis.

In: Biochemical and Biophysical Research Communications, Vol. 137, No. 3, 30.06.1986, p. 950-956.

Research output: Contribution to journalArticle

@article{af266ab47c96461aa4f982e5da8a27ea,
title = "Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture",
abstract = "Insulin-like growth factor-I (IGF-I) in concentration as low as 10 ng/ml significantly increased basal testosterone formation and 100 ng/ml of IGF-I increased testosterone production more than two fold in primary cultures of purified mature Leydig cells. IGF-I also markedly potentiated hCG-induced testosterone formation in a dose-dependent manner. Furthermore, IGF-I enhanced 8-bromo cyclic AMP induced steroidogenesis and hCG-stimulated cyclic AMP formation. The binding of 125I-IGF-I to purified Leydig cells was linear with a binding affinity of 0.56 ± 0.07×109 M-1 and a capacity of 167 ± 10.2 fmol/mg protein. Insulin and multiplication-stimulating activity were less potent than IGF-I in competing the binding of 125I-IGF-I to purified Leydig cells. This suggests that Leydig cells contain specific type I IGF receptor and IGF-I could modulate Leydig cell steroidogenesis.",
author = "T. Lin and J. Haskell and N. Vinson and Louis Terracio",
year = "1986",
month = "6",
day = "30",
doi = "10.1016/0006-291X(86)90317-7",
language = "English (US)",
volume = "137",
pages = "950--956",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture

AU - Lin, T.

AU - Haskell, J.

AU - Vinson, N.

AU - Terracio, Louis

PY - 1986/6/30

Y1 - 1986/6/30

N2 - Insulin-like growth factor-I (IGF-I) in concentration as low as 10 ng/ml significantly increased basal testosterone formation and 100 ng/ml of IGF-I increased testosterone production more than two fold in primary cultures of purified mature Leydig cells. IGF-I also markedly potentiated hCG-induced testosterone formation in a dose-dependent manner. Furthermore, IGF-I enhanced 8-bromo cyclic AMP induced steroidogenesis and hCG-stimulated cyclic AMP formation. The binding of 125I-IGF-I to purified Leydig cells was linear with a binding affinity of 0.56 ± 0.07×109 M-1 and a capacity of 167 ± 10.2 fmol/mg protein. Insulin and multiplication-stimulating activity were less potent than IGF-I in competing the binding of 125I-IGF-I to purified Leydig cells. This suggests that Leydig cells contain specific type I IGF receptor and IGF-I could modulate Leydig cell steroidogenesis.

AB - Insulin-like growth factor-I (IGF-I) in concentration as low as 10 ng/ml significantly increased basal testosterone formation and 100 ng/ml of IGF-I increased testosterone production more than two fold in primary cultures of purified mature Leydig cells. IGF-I also markedly potentiated hCG-induced testosterone formation in a dose-dependent manner. Furthermore, IGF-I enhanced 8-bromo cyclic AMP induced steroidogenesis and hCG-stimulated cyclic AMP formation. The binding of 125I-IGF-I to purified Leydig cells was linear with a binding affinity of 0.56 ± 0.07×109 M-1 and a capacity of 167 ± 10.2 fmol/mg protein. Insulin and multiplication-stimulating activity were less potent than IGF-I in competing the binding of 125I-IGF-I to purified Leydig cells. This suggests that Leydig cells contain specific type I IGF receptor and IGF-I could modulate Leydig cell steroidogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0022509111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022509111&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)90317-7

DO - 10.1016/0006-291X(86)90317-7

M3 - Article

VL - 137

SP - 950

EP - 956

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -