Direct evidence for SAA deposition in tissues during murine amyloidogenesis

Shoshana Yakar, B. Kaplan, A. Livneh, B. Martin, K. Miura, Z. Ali-Khan, S. Shtrasburg, M. Pras

Research output: Contribution to journalArticle

Abstract

To study the mechanism of amyloid deposition, the nature of amyloid proteins formed in experimental murine amyloidosis, was examined. Spleen specimens, 15-60 mg, were homogenized and extracted using aqueous acidic acetonitrile, in a recently developed procedure, making it possible to obtain amyloid proteins from minute amounts of tissue. The extracted material, 1.5-4 mg, was analysed by Western blotting and ELISA using antibodies recognizing differentially proteins AA and SAA. Two immunoreactive proteins of 8 and 12 KDa were isolated and subjected to amino acid analysis and N-terminal sequence determination. The results of immunochemical and chemical examination showed that the 8 and 12 KDa proteins represented proteins AA and SAA, respectively. The data obtained provide new direct evidence for SAA deposition in tissues during murine amyloidogenesis.

Original languageEnglish (US)
Pages (from-to)653-658
Number of pages6
JournalScandinavian Journal of Immunology
Volume40
Issue number6
DOIs
StatePublished - 1994

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Amyloidogenic Proteins
Proteins
Amyloidosis
Amyloid
Sequence Analysis
Spleen
Western Blotting
Enzyme-Linked Immunosorbent Assay
Amino Acids
Antibodies
acetonitrile

ASJC Scopus subject areas

  • Immunology

Cite this

Direct evidence for SAA deposition in tissues during murine amyloidogenesis. / Yakar, Shoshana; Kaplan, B.; Livneh, A.; Martin, B.; Miura, K.; Ali-Khan, Z.; Shtrasburg, S.; Pras, M.

In: Scandinavian Journal of Immunology, Vol. 40, No. 6, 1994, p. 653-658.

Research output: Contribution to journalArticle

Yakar, S, Kaplan, B, Livneh, A, Martin, B, Miura, K, Ali-Khan, Z, Shtrasburg, S & Pras, M 1994, 'Direct evidence for SAA deposition in tissues during murine amyloidogenesis', Scandinavian Journal of Immunology, vol. 40, no. 6, pp. 653-658. https://doi.org/10.1111/j.1365-3083.1994.tb03519.x
Yakar, Shoshana ; Kaplan, B. ; Livneh, A. ; Martin, B. ; Miura, K. ; Ali-Khan, Z. ; Shtrasburg, S. ; Pras, M. / Direct evidence for SAA deposition in tissues during murine amyloidogenesis. In: Scandinavian Journal of Immunology. 1994 ; Vol. 40, No. 6. pp. 653-658.
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AU - Ali-Khan, Z.

AU - Shtrasburg, S.

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