Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis

Giuseppina Cangiano, Antonio Mazzone, Loredana Baccigalupi, Rachele Isticato, Patrick Eichenberger, Maurilio De Felice, Ezio Ricca

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Abstract

GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by σE-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on σE-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.

Original languageEnglish (US)
Pages (from-to)3406-3413
Number of pages8
JournalJournal of bacteriology
Volume192
Issue number13
DOIs
StatePublished - Jul 1 2010

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cangiano, G., Mazzone, A., Baccigalupi, L., Isticato, R., Eichenberger, P., De Felice, M., & Ricca, E. (2010). Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis. Journal of bacteriology, 192(13), 3406-3413. https://doi.org/10.1128/JB.00329-10