Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis

Giuseppina Cangiano, Antonio Mazzone, Loredana Baccigalupi, Rachele Isticato, Patrick Eichenberger, Maurilio De Felice, Ezio Ricca

Research output: Contribution to journalArticle

Abstract

GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by σE-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on σE-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.

Original languageEnglish (US)
Pages (from-to)3406-3413
Number of pages8
JournalJournal of Bacteriology
Volume192
Issue number13
DOIs
StatePublished - Jul 2010

Fingerprint

Bacillus subtilis
Genes
DNA-Directed RNA Polymerases
Chromatin Immunoprecipitation
Regulator Genes
Genetic Promoter Regions

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cangiano, G., Mazzone, A., Baccigalupi, L., Isticato, R., Eichenberger, P., De Felice, M., & Ricca, E. (2010). Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis. Journal of Bacteriology, 192(13), 3406-3413. https://doi.org/10.1128/JB.00329-10

Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis. / Cangiano, Giuseppina; Mazzone, Antonio; Baccigalupi, Loredana; Isticato, Rachele; Eichenberger, Patrick; De Felice, Maurilio; Ricca, Ezio.

In: Journal of Bacteriology, Vol. 192, No. 13, 07.2010, p. 3406-3413.

Research output: Contribution to journalArticle

Cangiano, G, Mazzone, A, Baccigalupi, L, Isticato, R, Eichenberger, P, De Felice, M & Ricca, E 2010, 'Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis', Journal of Bacteriology, vol. 192, no. 13, pp. 3406-3413. https://doi.org/10.1128/JB.00329-10
Cangiano, Giuseppina ; Mazzone, Antonio ; Baccigalupi, Loredana ; Isticato, Rachele ; Eichenberger, Patrick ; De Felice, Maurilio ; Ricca, Ezio. / Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis. In: Journal of Bacteriology. 2010 ; Vol. 192, No. 13. pp. 3406-3413.
@article{2e7633fbfdca40938a439d9aa3674c74,
title = "Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis",
abstract = "GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by σE-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on σE-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.",
author = "Giuseppina Cangiano and Antonio Mazzone and Loredana Baccigalupi and Rachele Isticato and Patrick Eichenberger and {De Felice}, Maurilio and Ezio Ricca",
year = "2010",
month = "7",
doi = "10.1128/JB.00329-10",
language = "English (US)",
volume = "192",
pages = "3406--3413",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "13",

}

TY - JOUR

T1 - Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis

AU - Cangiano, Giuseppina

AU - Mazzone, Antonio

AU - Baccigalupi, Loredana

AU - Isticato, Rachele

AU - Eichenberger, Patrick

AU - De Felice, Maurilio

AU - Ricca, Ezio

PY - 2010/7

Y1 - 2010/7

N2 - GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by σE-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on σE-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.

AB - GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by σE-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on σE-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.

UR - http://www.scopus.com/inward/record.url?scp=77954368190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954368190&partnerID=8YFLogxK

U2 - 10.1128/JB.00329-10

DO - 10.1128/JB.00329-10

M3 - Article

VL - 192

SP - 3406

EP - 3413

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 13

ER -