Differential receptors create patterns diagnostic for ATP and GTP

Shawn C. McCleskey, Michael J. Griffin, Stephen E. Schneider, John McDevitt, Eric V. Anslyn

Research output: Contribution to journalArticle

Abstract

Herein we report the combination of a library of resin-bound sensors along with a multicomponent sensor array. This novel combinatorial array sensor system shows selectivity for nucleotide phosphates in solution. The design of the anchored receptor includes a 1,3,5-trisubstituted-2,4,6-triethylbenzene scaffold coupled with peptide libraries. Each chemosensor is placed into a micromachined cavity within a silicon wafer, and the optical changes observed by a charged-coupled device result in near-real-time digital analysis of solutions. A colorimetric displacement assay was performed, and time-dependent imaging studies of the selected sensing ensembles result in a differential responses upon addition of adenosine 5′-triphosphate (ATP), adenosine 5′-monophosphate (AMP), or guanosine 5′-triphosphate (GTP). An advantage to this approach is that it creates an array of sensors that gives a fingerprint response for each analyte. Principal component analysis indicates that the library of chemosensors can differentiate between ATP, GTP, and AMP. On the basis of factor loading values, individual sensors from the library were sequenced to elucidate their chemical composition.

Original languageEnglish (US)
Pages (from-to)1114-1115
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number5
DOIs
StatePublished - Feb 5 2003

Fingerprint

Guanosine Triphosphate
Libraries
Adenosine Triphosphate
Sensor arrays
Adenosine Monophosphate
Sensors
Peptide Library
Guanosine
Dermatoglyphics
Silicon
Principal Component Analysis
Silicon wafers
Scaffolds
Principal component analysis
Assays
Scaffolds (biology)
Nucleotides
Resins
Phosphates
Peptides

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Differential receptors create patterns diagnostic for ATP and GTP. / McCleskey, Shawn C.; Griffin, Michael J.; Schneider, Stephen E.; McDevitt, John; Anslyn, Eric V.

In: Journal of the American Chemical Society, Vol. 125, No. 5, 05.02.2003, p. 1114-1115.

Research output: Contribution to journalArticle

McCleskey, Shawn C. ; Griffin, Michael J. ; Schneider, Stephen E. ; McDevitt, John ; Anslyn, Eric V. / Differential receptors create patterns diagnostic for ATP and GTP. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 5. pp. 1114-1115.
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