Differential labeling with orotic acid and uridine in compensatory renal hypertrophy

J. S. Ross, Daniel Malamud, J. B. Caulfield, R. A. Malt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Using [5- 3H]orotic acid and [5- 3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5- 3H]orotic acid in tubules labeled RNA 15 times more intensely than [5- 3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5- 3H]uridine should be used as the precursor if generalized labeling is desired, but [5- 3H]orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology
Pages952-954
Number of pages3
Volume229
Edition4
StatePublished - 1975

Fingerprint

Orotic Acid
Uridine
Hypertrophy
Kidney
RNA
RNA Precursors
Nephrons

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ross, J. S., Malamud, D., Caulfield, J. B., & Malt, R. A. (1975). Differential labeling with orotic acid and uridine in compensatory renal hypertrophy. In American Journal of Physiology (4 ed., Vol. 229, pp. 952-954)

Differential labeling with orotic acid and uridine in compensatory renal hypertrophy. / Ross, J. S.; Malamud, Daniel; Caulfield, J. B.; Malt, R. A.

American Journal of Physiology. Vol. 229 4. ed. 1975. p. 952-954.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ross, JS, Malamud, D, Caulfield, JB & Malt, RA 1975, Differential labeling with orotic acid and uridine in compensatory renal hypertrophy. in American Journal of Physiology. 4 edn, vol. 229, pp. 952-954.
Ross JS, Malamud D, Caulfield JB, Malt RA. Differential labeling with orotic acid and uridine in compensatory renal hypertrophy. In American Journal of Physiology. 4 ed. Vol. 229. 1975. p. 952-954
Ross, J. S. ; Malamud, Daniel ; Caulfield, J. B. ; Malt, R. A. / Differential labeling with orotic acid and uridine in compensatory renal hypertrophy. American Journal of Physiology. Vol. 229 4. ed. 1975. pp. 952-954
@inbook{ebbb6c5f2b2644818177148644d676e1,
title = "Differential labeling with orotic acid and uridine in compensatory renal hypertrophy",
abstract = "Using [5- 3H]orotic acid and [5- 3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5- 3H]orotic acid in tubules labeled RNA 15 times more intensely than [5- 3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97{\%} was in tubular cells, mostly in the proximal tubules. Only about 80{\%} of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5- 3H]uridine should be used as the precursor if generalized labeling is desired, but [5- 3H]orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.",
author = "Ross, {J. S.} and Daniel Malamud and Caulfield, {J. B.} and Malt, {R. A.}",
year = "1975",
language = "English (US)",
volume = "229",
pages = "952--954",
booktitle = "American Journal of Physiology",
edition = "4",

}

TY - CHAP

T1 - Differential labeling with orotic acid and uridine in compensatory renal hypertrophy

AU - Ross, J. S.

AU - Malamud, Daniel

AU - Caulfield, J. B.

AU - Malt, R. A.

PY - 1975

Y1 - 1975

N2 - Using [5- 3H]orotic acid and [5- 3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5- 3H]orotic acid in tubules labeled RNA 15 times more intensely than [5- 3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5- 3H]uridine should be used as the precursor if generalized labeling is desired, but [5- 3H]orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

AB - Using [5- 3H]orotic acid and [5- 3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5- 3H]orotic acid in tubules labeled RNA 15 times more intensely than [5- 3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5- 3H]uridine should be used as the precursor if generalized labeling is desired, but [5- 3H]orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

UR - http://www.scopus.com/inward/record.url?scp=0016685586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016685586&partnerID=8YFLogxK

M3 - Chapter

VL - 229

SP - 952

EP - 954

BT - American Journal of Physiology

ER -