Differential expression of IGF-I and insulin receptor lsoforms in HPV positive and negative human cervical cancer cell lines

M. L. Serrano, M. Sánchez-Gómez, M. M. Bravo, Shoshana Yakar, D. LeRoith

Research output: Contribution to journalArticle

Abstract

Human papillomavirus (HPV) is the main risk factor for cervical cancer; however, some carcinomas occur in the absence of the virus. IGF-IR and an isoform of the insulin receptor, IR-A, play important roles in cancer. In this study we assessed the role of the IGF/insulin receptors in cervical cancer cell lines with different HPV status, SiHa (HPV positive), and C33a (HPV negative). Different patterns of receptor expression were found; while SiHa expressed IGF-IR, IR-A and IR-B, and IR/IGF-IR hybrid receptors, C33a cells expressed the IR-A only. Tyrosine phosphorylation of these receptors in response to their corresponding ligands correlated with the expression level of these receptors in the cell lines. Activation of PI3-K and MAPK pathways was revealed in both cell lines, however, no effects on proliferation, migration, or invasion were observed. Here we show that cervical cancer cell lines - positive and negative for HPV - differ in the type of insulin and IGF-1 receptors expressed. Additional studies are needed for characterization of the role of IR-A in cervical carcinogenesis.

Original languageEnglish (US)
Pages (from-to)661-667
Number of pages7
JournalHormone and Metabolic Research
Volume40
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

IGF Type 1 Receptor
Insulin Receptor
Uterine Cervical Neoplasms
Cells
Cell Line
Phosphorylation
Viruses
Tyrosine
Protein Isoforms
Chemical activation
Insulin
Ligands
Carcinogenesis
Carcinoma
Neoplasms

Keywords

  • C33a
  • Human papillomavirus (HPV)
  • Insulin receptor (IR)
  • Insulin-like growth factor-I receptor (IGF-IR)
  • IR-A
  • SiHa

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential expression of IGF-I and insulin receptor lsoforms in HPV positive and negative human cervical cancer cell lines. / Serrano, M. L.; Sánchez-Gómez, M.; Bravo, M. M.; Yakar, Shoshana; LeRoith, D.

In: Hormone and Metabolic Research, Vol. 40, No. 10, 10.2008, p. 661-667.

Research output: Contribution to journalArticle

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