Different serotonergic expression in nevomelanocytic tumors

Clara Naimi-Akbar, Markus Ritter, Sasika Demel, Husameldin El-Nour, Mari Anne Hedblad, Efrain Azmitia, Klas Nordlind

Research output: Contribution to journalArticle

Abstract

The neuromediator serotonin (5-hydroxytryptamine; 5-HT) has been proposed to play a role in tumor progression. Thus, the aim of the present investigation was to determine whether alterations in the serotonergic system occur in nevomelanocytic tumors. For this purpose, paraffin-embedded biopsies of superficial spreading malignant melanoma (SSM), dysplastic compound nevi (DN) and benign compound nevi (BCN) were characterized with regard to their expression of 5-HT, the 5-HT1A and 5-HT2A receptors, and the serotonin transporter protein (SERT), by immunohistochemical analysis. Melanocytes in the region surrounding the tumor were found to express both the 5-HT1A and 5-HT2A receptors. Tumor cells that immunostained positively for the different serotonergic markers were observed in the suprabasal epidermis of DN tissue and, to an even greater extent, in the case of SSM. Furthermore, some of these latter cells expressed both 5-HT1AR and 5-HT2AR. The level of expression of 5-HT1AR at the junctional area was lower for SSM than for DN or BCN. As the degree of atypia increased, the intensity of tumor cell staining in the dermis for 5-HT1AR and SERT declined. Vessel immunoreactivity for 5-HT2A was more intense in SSM than in BCN tissue. Round-to-dendritic cells that expressed both SERT and 5-HT1AR were seen to infiltrate into the dermal region of the tumor, this infiltration being more evident in the case of DN and SSM. These latter cells were also tryptase-positive, indicating that they are mast cells. Thus, alterations in serotonergic system may be involved in nevomelanocytic tumors and mast cells may play an important role in this connection.

Original languageEnglish (US)
Pages (from-to)1166-1177
Number of pages12
JournalCancers
Volume2
Issue number2
DOIs
StatePublished - Jun 2010

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Dysplastic Nevus Syndrome
Melanoma
Serotonin Plasma Membrane Transport Proteins
Nevus
Serotonin
Neoplasms
Receptor, Serotonin, 5-HT2A
Mast Cells
Tryptases
Proteins
Melanocytes
Dermis
Epidermis
Paraffin
Dendritic Cells
Staining and Labeling
Biopsy
Skin

Keywords

  • Immunohistochemistry
  • Melanoma
  • Receptor
  • Serotonin
  • Serotonin transporter protein

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Naimi-Akbar, C., Ritter, M., Demel, S., El-Nour, H., Hedblad, M. A., Azmitia, E., & Nordlind, K. (2010). Different serotonergic expression in nevomelanocytic tumors. Cancers, 2(2), 1166-1177. https://doi.org/10.3390/cancers2021166

Different serotonergic expression in nevomelanocytic tumors. / Naimi-Akbar, Clara; Ritter, Markus; Demel, Sasika; El-Nour, Husameldin; Hedblad, Mari Anne; Azmitia, Efrain; Nordlind, Klas.

In: Cancers, Vol. 2, No. 2, 06.2010, p. 1166-1177.

Research output: Contribution to journalArticle

Naimi-Akbar, C, Ritter, M, Demel, S, El-Nour, H, Hedblad, MA, Azmitia, E & Nordlind, K 2010, 'Different serotonergic expression in nevomelanocytic tumors', Cancers, vol. 2, no. 2, pp. 1166-1177. https://doi.org/10.3390/cancers2021166
Naimi-Akbar C, Ritter M, Demel S, El-Nour H, Hedblad MA, Azmitia E et al. Different serotonergic expression in nevomelanocytic tumors. Cancers. 2010 Jun;2(2):1166-1177. https://doi.org/10.3390/cancers2021166
Naimi-Akbar, Clara ; Ritter, Markus ; Demel, Sasika ; El-Nour, Husameldin ; Hedblad, Mari Anne ; Azmitia, Efrain ; Nordlind, Klas. / Different serotonergic expression in nevomelanocytic tumors. In: Cancers. 2010 ; Vol. 2, No. 2. pp. 1166-1177.
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