Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes

Yuqin Cai, Konstantin Kropachev, Michael A. Terzidis, Annalisa Masi, Chryssostomos Chatgilialoglu, Vladimir Shafirovich, Nicholas Geacintov, Suse Broyde

Research output: Contribution to journalArticle

Abstract

In nucleosomes, the access of DNA lesions to nucleotide excision repair is hindered by histone proteins. However, evidence that the nature of the DNA lesions may play a role in facilitating access is emerging, but these phenomena are not well-understood. We have used molecular dynamics simulations to elucidate the structural, dynamic, and energetic properties of the R and S 5′-8-cyclo-2′-dG and the (+)-cis-anti-B[a]P-dG lesions in a nucleosome. Our results show that the (+)-cis-anti-B[a]P-dG adduct is more dynamic and more destabilizing than the smaller and more constrained 5′,8-cyclo-2′-dG lesions, suggesting more facile access to the more bulky (+)-cis-anti-B[a]P-dG lesion.

Original languageEnglish (US)
Pages (from-to)4181-4185
Number of pages5
JournalBiochemistry
Volume54
Issue number27
DOIs
StatePublished - Jul 14 2015

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Nucleosomes
DNA Repair
Machinery
Repair
Nucleotides
DNA
Structural dynamics
Molecular Dynamics Simulation
Histones
Molecular dynamics
Computer simulation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cai, Y., Kropachev, K., Terzidis, M. A., Masi, A., Chatgilialoglu, C., Shafirovich, V., ... Broyde, S. (2015). Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes. Biochemistry, 54(27), 4181-4185. https://doi.org/10.1021/acs.biochem.5b00564

Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes. / Cai, Yuqin; Kropachev, Konstantin; Terzidis, Michael A.; Masi, Annalisa; Chatgilialoglu, Chryssostomos; Shafirovich, Vladimir; Geacintov, Nicholas; Broyde, Suse.

In: Biochemistry, Vol. 54, No. 27, 14.07.2015, p. 4181-4185.

Research output: Contribution to journalArticle

Cai, Y, Kropachev, K, Terzidis, MA, Masi, A, Chatgilialoglu, C, Shafirovich, V, Geacintov, N & Broyde, S 2015, 'Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes', Biochemistry, vol. 54, no. 27, pp. 4181-4185. https://doi.org/10.1021/acs.biochem.5b00564
Cai Y, Kropachev K, Terzidis MA, Masi A, Chatgilialoglu C, Shafirovich V et al. Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes. Biochemistry. 2015 Jul 14;54(27):4181-4185. https://doi.org/10.1021/acs.biochem.5b00564
Cai, Yuqin ; Kropachev, Konstantin ; Terzidis, Michael A. ; Masi, Annalisa ; Chatgilialoglu, Chryssostomos ; Shafirovich, Vladimir ; Geacintov, Nicholas ; Broyde, Suse. / Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes. In: Biochemistry. 2015 ; Vol. 54, No. 27. pp. 4181-4185.
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