Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids

Rastislav Levicky, Ursula Koniges, Napoleon Tercero

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Diagnostic applications of morpholinos take advantage of their unique properties including backbone charge neutrality, a weak impact of ionic strength on their hybridization behavior, and their resistance to enzymatic degradation. This chapter overviews how these properties have advanced transduction and other capabilities useful for the analysis of nucleic acids. In many cases, the benefits stem from electrostatic mechanisms; for example, use of low ionic strengths improves sensitivity of detection while decreasing background signals because only the nucleic acid analyte is charged. While most literature reports focus on in vitro assays in buffer, morpholinos have been also used for biodistribution measurements of species such as fungal rRNA and miRNA. After reviewing the diagnostic applications of morpholinos, the chapter describes preparation of morpholino monolayers on metal supports for electrochemical diagnostics and the procedure for performing label-free detection of DNA from changes in surface capacitance.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-190
Number of pages10
Volume1565
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1565
ISSN (Print)10643745

Fingerprint

Morpholinos
Nucleic Acids
Osmolar Concentration
Static Electricity
MicroRNAs
Buffers
Metals
DNA

Keywords

  • Biosensor
  • FISH
  • Fluorescence
  • Imaging
  • Interface
  • Microarray
  • Surface

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Levicky, R., Koniges, U., & Tercero, N. (2017). Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids. In Methods in Molecular Biology (Vol. 1565, pp. 181-190). (Methods in Molecular Biology; Vol. 1565). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6817-6_15

Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids. / Levicky, Rastislav; Koniges, Ursula; Tercero, Napoleon.

Methods in Molecular Biology. Vol. 1565 Humana Press Inc., 2017. p. 181-190 (Methods in Molecular Biology; Vol. 1565).

Research output: Chapter in Book/Report/Conference proceedingChapter

Levicky, R, Koniges, U & Tercero, N 2017, Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids. in Methods in Molecular Biology. vol. 1565, Methods in Molecular Biology, vol. 1565, Humana Press Inc., pp. 181-190. https://doi.org/10.1007/978-1-4939-6817-6_15
Levicky R, Koniges U, Tercero N. Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids. In Methods in Molecular Biology. Vol. 1565. Humana Press Inc. 2017. p. 181-190. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6817-6_15
Levicky, Rastislav ; Koniges, Ursula ; Tercero, Napoleon. / Diagnostic applications of morpholinos and label-free electrochemical detection of nucleic acids. Methods in Molecular Biology. Vol. 1565 Humana Press Inc., 2017. pp. 181-190 (Methods in Molecular Biology).
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