Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans

Ping Chen, Jan Novak, Fengxia Qi, Page W. Caufield

Research output: Contribution to journalArticle

Abstract

Genetic characterization of a Tn916 transposon mutant, Streptococcus mutans TS-1, defective in mutacin II production, revealed that the transposon was inserted into the 3' region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S. mutans mutant, GS5Tn1, which was altered in its ability to respond to environmental stress (Y. Yamashita, T. Takehara, and H. K. Kuramitsu, J. Bacteriol. 175:6220-6228, 1993). Quantitative primer extension from the mutacin structural gene mutA showed a decreased level (about eightfold) of mut A transcription for mutant T8-1. Mutacin production was restored by transforming mutant T8-1 with integration vector pVA891 containing an intact dgk gene. These data indicated that the full-length dgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalJournal of Bacteriology
Volume180
Issue number1
StatePublished - Jan 1998

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Diacylglycerol Kinase
Bacteriocins
Streptococcus mutans
Genes
Operon
mutacin II

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans. / Chen, Ping; Novak, Jan; Qi, Fengxia; Caufield, Page W.

In: Journal of Bacteriology, Vol. 180, No. 1, 01.1998, p. 167-170.

Research output: Contribution to journalArticle

Chen, Ping ; Novak, Jan ; Qi, Fengxia ; Caufield, Page W. / Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 1. pp. 167-170.
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