Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene

Jhumku D. Kohtz, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

We previously reported that sonic hedgehog (Shh) induces the differentiation of rat ventral forebrain neurons expressing a novel marker, EVF-1 [Development 125 (1998) 5079]. In this report, we show that EVF-1 is a novel, developmentally regulated, non-coding RNA, with no homology to other known non-coding RNA sequences. Sequence analysis, in vitro translation, and comparison of the rat and mouse EVF-1 sequences suggest that EVF-1 contains no protein coding regions. Chromosomal location indicates that EVF-1 maps adjacent to the Dlx6 gene on mouse chromosome 6. RNA in situ hybridization of the embryonic rat forebrain shows that EVF-1 is expressed by immature neurons in the subventricular zone and its expression decreases during forebrain development. Whole mount in situ hybridization shows that EVF-1 is expressed at high levels in the branchial arches, ventral forebrain, olfactory bulb, and limbs. EVF-1 expression is linked to Shh and the Dlx family of proteins, genes with a demonstrated importance to ventral forebrain and craniofacial development.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalGene Expression Patterns
Volume4
Issue number4
DOIs
StatePublished - Jul 1 2004

Fingerprint

Untranslated RNA
Prosencephalon
Hedgehogs
Genes
In Situ Hybridization
Branchial Region
Neurons
Chromosomes, Human, Pair 6
Lateral Ventricles
Olfactory Bulb
Open Reading Frames
Sequence Analysis
Extremities
RNA

Keywords

  • Differentiation
  • Dlx genes
  • Forebrain development
  • Homeodomain
  • Immature neurons
  • Non-coding RNA
  • Nuclear RNA
  • Sonic hedgehog
  • Telencephalon
  • Ventral forebrain

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene. / Kohtz, Jhumku D.; Fishell, Gordon.

In: Gene Expression Patterns, Vol. 4, No. 4, 01.07.2004, p. 407-412.

Research output: Contribution to journalArticle

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