Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis

Anna Di Gregorio, Maria Grazia Villani, Annamaria Locascio, Filomena Ristoratore, Francesco Aniello, Margherita Branno

Research output: Contribution to journalArticle

Abstract

A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.

Original languageEnglish (US)
Pages (from-to)387-394
Number of pages8
JournalDevelopment Growth and Differentiation
Volume40
Issue number4
StatePublished - 1998

Fingerprint

Calmodulin
Ciona intestinalis
Messenger RNA
Larva
Neural Plate
Urochordata
Gene Library
Northern Blotting
Nervous System
Open Reading Frames
In Situ Hybridization
Sequence Analysis
Tail
Embryonic Structures
Complementary DNA
Molecular Weight
Amino Acids
Proteins

Keywords

  • Ascidian
  • Calmodulin
  • Ciona intestinalis

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis. / Di Gregorio, Anna; Villani, Maria Grazia; Locascio, Annamaria; Ristoratore, Filomena; Aniello, Francesco; Branno, Margherita.

In: Development Growth and Differentiation, Vol. 40, No. 4, 1998, p. 387-394.

Research output: Contribution to journalArticle

Di Gregorio, Anna ; Villani, Maria Grazia ; Locascio, Annamaria ; Ristoratore, Filomena ; Aniello, Francesco ; Branno, Margherita. / Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis. In: Development Growth and Differentiation. 1998 ; Vol. 40, No. 4. pp. 387-394.
@article{29973945c23f4e2b8a86a9661fe7a82d,
title = "Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis",
abstract = "A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98{\%} identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.",
keywords = "Ascidian, Calmodulin, Ciona intestinalis",
author = "{Di Gregorio}, Anna and Villani, {Maria Grazia} and Annamaria Locascio and Filomena Ristoratore and Francesco Aniello and Margherita Branno",
year = "1998",
language = "English (US)",
volume = "40",
pages = "387--394",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis

AU - Di Gregorio, Anna

AU - Villani, Maria Grazia

AU - Locascio, Annamaria

AU - Ristoratore, Filomena

AU - Aniello, Francesco

AU - Branno, Margherita

PY - 1998

Y1 - 1998

N2 - A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.

AB - A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.

KW - Ascidian

KW - Calmodulin

KW - Ciona intestinalis

UR - http://www.scopus.com/inward/record.url?scp=0031846636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031846636&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 387

EP - 394

JO - Development Growth and Differentiation

JF - Development Growth and Differentiation

SN - 0012-1592

IS - 4

ER -