Developmental microglial priming in postmortem autism spectrum disorder temporal cortex

Andrew S. Lee, Efrain Azmitia, Patricia M. Whitaker-Azmitia

Research output: Contribution to journalArticle

Abstract

Microglia can shift into different complex morphologies depending on the microenvironment of the central nervous system (CNS). The distinct morphologies correlate with specific functions and can indicate the pathophysiological state of the CNS. Previous postmortem studies of autism spectrum disorder (ASD) showed neuroinflammation in ASD indicated by increased microglial density. These changes in the microglia density can be accompanied by changes in microglia phenotype but the individual contribution of different microglia phenotypes to the pathophysiology of ASD remains unclear. Here, we used an unbiased stereological approach to quantify six structurally and functionally distinct microglia phenotypes in postmortem human temporal cortex, which were immuno-stained with Iba1. The total density of all microglia phenotypes did not differ between ASD donors and typically developing individual donors. However, there was a significant decrease in ramified microglia in both gray matter and white matter of ASD, and a significant increase in primed microglia in gray matter of ASD compared to typically developing individuals. This increase in primed microglia showed a positive correlation with donor age in both gray matter and white of ASD, but not in typically developing individuals. Our results provide evidence of a shift in microglial phenotype that may indicate impaired synaptic plasticity and a chronic vulnerability to exaggerated immune responses.

Original languageEnglish (US)
JournalBrain, Behavior, and Immunity
DOIs
StateAccepted/In press - Nov 9 2016

Fingerprint

Microglia
Temporal Lobe
Phenotype
Central Nervous System
Autism Spectrum Disorder
Neuronal Plasticity

Keywords

  • Autism spectrum disorder
  • Developmental priming
  • Microglia
  • Neuroinflammation
  • Postmortem human brain
  • Temporal cortex

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Developmental microglial priming in postmortem autism spectrum disorder temporal cortex. / Lee, Andrew S.; Azmitia, Efrain; Whitaker-Azmitia, Patricia M.

In: Brain, Behavior, and Immunity, 09.11.2016.

Research output: Contribution to journalArticle

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