Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex

Margaret Jia, Alessio Travaglia, Gabriella Pollonini, Giuseppe Fedele, Cristina Alberini

Research output: Contribution to journalArticle

Abstract

The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages.

Original languageEnglish (US)
Pages (from-to)533-543
Number of pages11
JournalLearning & memory (Cold Spring Harbor, N.Y.)
Volume25
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

Neuronal Plasticity
Prefrontal Cortex
Neuroglia
Proteins
Long-Term Memory
Aptitude
Brain
Synapses
Decision Making

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex. / Jia, Margaret; Travaglia, Alessio; Pollonini, Gabriella; Fedele, Giuseppe; Alberini, Cristina.

In: Learning & memory (Cold Spring Harbor, N.Y.), Vol. 25, No. 10, 01.10.2018, p. 533-543.

Research output: Contribution to journalArticle

Jia, Margaret ; Travaglia, Alessio ; Pollonini, Gabriella ; Fedele, Giuseppe ; Alberini, Cristina. / Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex. In: Learning & memory (Cold Spring Harbor, N.Y.). 2018 ; Vol. 25, No. 10. pp. 533-543.
@article{4a2a674f149441b296319108a0720260,
title = "Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex",
abstract = "The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages.",
author = "Margaret Jia and Alessio Travaglia and Gabriella Pollonini and Giuseppe Fedele and Cristina Alberini",
year = "2018",
month = "10",
day = "1",
doi = "10.1101/lm.047753.118",
language = "English (US)",
volume = "25",
pages = "533--543",
journal = "Learning and Memory",
issn = "1072-0502",
publisher = "Cold Spring Harbor Laboratory Press",
number = "10",

}

TY - JOUR

T1 - Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex

AU - Jia, Margaret

AU - Travaglia, Alessio

AU - Pollonini, Gabriella

AU - Fedele, Giuseppe

AU - Alberini, Cristina

PY - 2018/10/1

Y1 - 2018/10/1

N2 - The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages.

AB - The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages.

UR - http://www.scopus.com/inward/record.url?scp=85054062077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054062077&partnerID=8YFLogxK

U2 - 10.1101/lm.047753.118

DO - 10.1101/lm.047753.118

M3 - Article

VL - 25

SP - 533

EP - 543

JO - Learning and Memory

JF - Learning and Memory

SN - 1072-0502

IS - 10

ER -