Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study

Mark Wolff, L. Mirels, J. Lagner, A. R. Hand

Research output: Contribution to journalArticle

Abstract

Cell differentiation in the rat sublingual gland occurs rapidly and is largely complete by birth. To study differentiation of the serous and mucous cells of the sublingual gland, we used antibodies to the secretory proteins CSP-1, SMGB, PSP, and SMGD, and sublingual mucin as specific cell markers. Glands from rats at ages 18, 19, and 20 days in utero, and postnatal days 0, 1, 5, 9, 14, 18, 25, 40, and 60 were fixed and prepared for morphological analysis and immunocytochemical labeling. At age 18 days in utero, a few cells in the developing terminal bulbs contained mucous-like apical granules that labeled with anti-mucin. Other cells had mixed granules with a peripheral lucent region and a dense core of variable size that occasionally labeled with anti-SMGD. Additionally, presumptive serous cells with small dense granules that contained CSP-1 and SMGB were present. At age 19 days in utero, the dense granules of these cells also labeled with anti-SMGD. By age 20 days in utero, mucous cells were filled with large, pale granules that labeled with anti-mucin, and serous cells had numerous dense granules containing CSP-1, SMGB, PSP, and SMGD. Fewer cells with mixed granules were seen, but dense regions present in some mucous granules (MGs) labeled with anti-SMGD. After birth, fewer MGs had dense regions, and serous cells were organized into well-formed demilunes. Except for PSP, which was undetectable after the fifth postnatal day, the pattern of immunoreactivity observed in glands of neonatal and adult animals was similar to that seen by age 20 days in utero. These results suggest that mucous and serous cells have separate developmental origins, mucous cells differentiate earlier than serous cells, and cells with mixed granules may become mucous cells.

Original languageEnglish (US)
Pages (from-to)30-42
Number of pages13
JournalAnatomical Record
Volume266
Issue number1
DOIs
StatePublished - Jan 1 2002

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Sublingual Gland
electrons
Electrons
Light
electron
granules
rats
cells
peripheral region
mucins
Mucins
antibody
protein
animal
Parturition
Newborn Animals

Keywords

  • Cell differentiation
  • Immunofluorescence
  • Immunogold labeling
  • Immunogold silver stain
  • Mucous acinar cells
  • Secretory proteins
  • Serous demilune cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Development of the rat sublingual gland : A light and electron microscopic immunocytochemical study. / Wolff, Mark; Mirels, L.; Lagner, J.; Hand, A. R.

In: Anatomical Record, Vol. 266, No. 1, 01.01.2002, p. 30-42.

Research output: Contribution to journalArticle

Wolff, Mark ; Mirels, L. ; Lagner, J. ; Hand, A. R. / Development of the rat sublingual gland : A light and electron microscopic immunocytochemical study. In: Anatomical Record. 2002 ; Vol. 266, No. 1. pp. 30-42.
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