Development of multianalyte sensor arrays composed of chemically derivatized polymeric microspheres localized in micromachined cavities

A. Goodey, J. J. Lavigne, S. M. Savoy, M. D. Rodriguez, T. Curey, A. Tsao, G. Simmons, J. Wright, S. J. Yoo, Y. Sohn, E. V. Anslyn, J. B. Shear, D. P. Neikirk, John McDevitt

Research output: Contribution to journalArticle

Abstract

The development of a chip-based sensor array composed of individually addressable polystyrene-poly(ethylene glycol) and agarose microspheres has been demonstrated. The microspheres are selectively arranged in micromachined cavities localized on silicon wafers. These cavities are created with an anisotropic etch and serve as miniaturized reaction vessels and analysis chambers. A single drop of fluid provides sufficient analysis media to complete ∼100 assays in these microetch pits. The cavities possess pyramidal pit shapes with trans-wafer openings that allows for both fluid flow through the microreactors/analysis chambers and optical access to the chemically sensitive microspheres. Identification and quantitation of analytes occurs via colorimetric and fluorescence changes to receptor and indicator molecules that are covalently attached to termination sites on the polymeric microspheres. Spectral data are extracted from the array efficiently using a charge-coupled device allowing for the near-real-time digital analysis of complex fluids. The power and utility of this new microbead array detection methodology is demonstrated here for the analysis of complex fluids containing a variety of important classes of analytes including acids, bases, metal cations, metabolic cofactors, and antibody reagents.

Original languageEnglish (US)
Pages (from-to)2559-2570
Number of pages12
JournalJournal of the American Chemical Society
Volume123
Issue number11
DOIs
StatePublished - 2001

Fingerprint

Sensor arrays
Microspheres
Fluids
Polystyrenes
Silicon wafers
Charge coupled devices
Antibodies
Sepharose
Ethylene Glycol
Polyethylene glycols
Cations
Flow of fluids
Silicon
Assays
Positive ions
Metals
Fluorescence
Molecules
Acids
Equipment and Supplies

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Development of multianalyte sensor arrays composed of chemically derivatized polymeric microspheres localized in micromachined cavities. / Goodey, A.; Lavigne, J. J.; Savoy, S. M.; Rodriguez, M. D.; Curey, T.; Tsao, A.; Simmons, G.; Wright, J.; Yoo, S. J.; Sohn, Y.; Anslyn, E. V.; Shear, J. B.; Neikirk, D. P.; McDevitt, John.

In: Journal of the American Chemical Society, Vol. 123, No. 11, 2001, p. 2559-2570.

Research output: Contribution to journalArticle

Goodey, A, Lavigne, JJ, Savoy, SM, Rodriguez, MD, Curey, T, Tsao, A, Simmons, G, Wright, J, Yoo, SJ, Sohn, Y, Anslyn, EV, Shear, JB, Neikirk, DP & McDevitt, J 2001, 'Development of multianalyte sensor arrays composed of chemically derivatized polymeric microspheres localized in micromachined cavities', Journal of the American Chemical Society, vol. 123, no. 11, pp. 2559-2570. https://doi.org/10.1021/ja003341l
Goodey, A. ; Lavigne, J. J. ; Savoy, S. M. ; Rodriguez, M. D. ; Curey, T. ; Tsao, A. ; Simmons, G. ; Wright, J. ; Yoo, S. J. ; Sohn, Y. ; Anslyn, E. V. ; Shear, J. B. ; Neikirk, D. P. ; McDevitt, John. / Development of multianalyte sensor arrays composed of chemically derivatized polymeric microspheres localized in micromachined cavities. In: Journal of the American Chemical Society. 2001 ; Vol. 123, No. 11. pp. 2559-2570.
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