Development of an in vitro model of neuronal activity induced excitotoxicity using photoconductive stimulation

Evgeny Pavlov, R. Carolina Gutiérrez, Yuan Zhang, Audrey C. Kertesz, Johanna Hung, Ferdinand Joseph Espina, Michael A. Colicos

Research output: Contribution to journalArticle

Abstract

Loss of the ability to regulate calcium is a central event leading to neuronal cell death during a wide range of pathological conditions including stroke and seizure. Here we present a new dissociated hippocampal cell culture model of acute electrical activity which incorporates the photoconductive stimulation of neuronal networks grown on silicon wafers. This technology allows precise modeling of user defined neuronal activity patterns, and the study of their effect on neuronal physiology. Here, seizure-like conditions were created by continuous stimulation, causing hundreds of neurons to fire synchronously at 50. Hz for 4. min. This stimulation protocol induced cell death as monitored by propidium iodide staining. The number of dead cells per stimulation region increased from 3.6 ± 2.1 preceding stimulation to 81 ± 21 30. min following stimulation. Excitotoxicity primarily affected excitatory rather than inhibitory neurons, and was preceded by an increase in intracellular calcium as well as changes in the mitochondrial membrane potential, as measured by a tetramethylrhodamine methyl ester (TMRM) assay. Cyclosporin A (CsA), a mitochondrial permeability transition pore (PTP) blocker, was effective in preventing cell death. We propose that photoconductive stimulation is a useful tool for investigating the pathogenesis of excitotoxicity in vitro.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalCell Calcium
Volume47
Issue number5
DOIs
StatePublished - May 2010

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Keywords

  • Cell death
  • Cyclosporin A
  • Excitotoxicity
  • Mitochondria
  • Neuron
  • Permeability transition

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Pavlov, E., Gutiérrez, R. C., Zhang, Y., Kertesz, A. C., Hung, J., Espina, F. J., & Colicos, M. A. (2010). Development of an in vitro model of neuronal activity induced excitotoxicity using photoconductive stimulation. Cell Calcium, 47(5), 441-448. https://doi.org/10.1016/j.ceca.2010.03.005