Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT)

William R. Abrams, Cheryl A. Barber, Kurt McCann, Gary Tong, Zongyuan Chen, Michael G. Mauk, Jing Wang, Alex Volkov, Pete Bourdelle, Paul L A M Corstjens, Michel Zuiderwijk, Keith Kardos, Shang Li, Hans J. Tanke, R. Sam Niedbala, Daniel Malamud, Haim Bau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Confirmatory detection of diseases, such as HIV and HIV-associated pathogens in a rapid point-of-care (POC) diagnostic remains a goal for disease control, prevention, and therapy. If a sample could be analyzed onsite with a verified result, the individual could be counseled immediately and appropriate therapy initiated. Our group is focused on developing a microfluidic "lab-on-a-chip" that will simultaneously identify antigens, antibodies, RNA, and DNA using a single oral sample. The approach has been to design individual modules for each assay that uses similar components (e.g., valves, heaters, metering chambers, mixers) installed on a polycarbonate base with a common reporter system. Assay miniaturization reduces the overall analysis time, increases accuracy by simultaneously identifying multiple targets, and enhances detector sensitivity by upconverting phosphor technology (UPT). Our microfluidic approach employs four interrelated components: (1) sample acquisition-OraSure UPlink™ collectors that pick-up and release bacteria, soluble analytes, and viruses from an oral sample; (2) microfluidic processing-movement of microliter volumes of analyte, target analyte extraction and amplification; (3) detection of analytes using UPT particles in a lateral flow system; and (4) software for processing the results. Ultimately, the oral-based microscale diagnostic system will detect viruses and bacteria, associated pathogen antigens and nucleic acids, and antibodies to these pathogens.

Original languageEnglish (US)
Title of host publicationOral-based Diagnostics
Pages375-388
Number of pages14
Volume1098
DOIs
StatePublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1098
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Pathogens
Phosphors
polycarbonate
Technology
Viruses
Assays
Bacteria
Point-of-Care Systems
HIV
Disease control
Miniaturization
Antigens
Lab-on-a-chip
Antibodies
Processing
Nucleic Acids
Amplification
Software

Keywords

  • Confirmatory
  • Diagnostic
  • HIV
  • Lateral flow
  • Microfluidic
  • Multiplex analysis
  • Oral fluid
  • Pathogen
  • Point-of-care
  • Saliva

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Abrams, W. R., Barber, C. A., McCann, K., Tong, G., Chen, Z., Mauk, M. G., ... Bau, H. (2007). Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT). In Oral-based Diagnostics (Vol. 1098, pp. 375-388). (Annals of the New York Academy of Sciences; Vol. 1098). https://doi.org/10.1196/annals.1384.020

Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT). / Abrams, William R.; Barber, Cheryl A.; McCann, Kurt; Tong, Gary; Chen, Zongyuan; Mauk, Michael G.; Wang, Jing; Volkov, Alex; Bourdelle, Pete; Corstjens, Paul L A M; Zuiderwijk, Michel; Kardos, Keith; Li, Shang; Tanke, Hans J.; Niedbala, R. Sam; Malamud, Daniel; Bau, Haim.

Oral-based Diagnostics. Vol. 1098 2007. p. 375-388 (Annals of the New York Academy of Sciences; Vol. 1098).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abrams, WR, Barber, CA, McCann, K, Tong, G, Chen, Z, Mauk, MG, Wang, J, Volkov, A, Bourdelle, P, Corstjens, PLAM, Zuiderwijk, M, Kardos, K, Li, S, Tanke, HJ, Niedbala, RS, Malamud, D & Bau, H 2007, Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT). in Oral-based Diagnostics. vol. 1098, Annals of the New York Academy of Sciences, vol. 1098, pp. 375-388. https://doi.org/10.1196/annals.1384.020
Abrams WR, Barber CA, McCann K, Tong G, Chen Z, Mauk MG et al. Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT). In Oral-based Diagnostics. Vol. 1098. 2007. p. 375-388. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1384.020
Abrams, William R. ; Barber, Cheryl A. ; McCann, Kurt ; Tong, Gary ; Chen, Zongyuan ; Mauk, Michael G. ; Wang, Jing ; Volkov, Alex ; Bourdelle, Pete ; Corstjens, Paul L A M ; Zuiderwijk, Michel ; Kardos, Keith ; Li, Shang ; Tanke, Hans J. ; Niedbala, R. Sam ; Malamud, Daniel ; Bau, Haim. / Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT). Oral-based Diagnostics. Vol. 1098 2007. pp. 375-388 (Annals of the New York Academy of Sciences).
@inproceedings{7aac04345d494e47afe67f88f98c9716,
title = "Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT)",
abstract = "Confirmatory detection of diseases, such as HIV and HIV-associated pathogens in a rapid point-of-care (POC) diagnostic remains a goal for disease control, prevention, and therapy. If a sample could be analyzed onsite with a verified result, the individual could be counseled immediately and appropriate therapy initiated. Our group is focused on developing a microfluidic {"}lab-on-a-chip{"} that will simultaneously identify antigens, antibodies, RNA, and DNA using a single oral sample. The approach has been to design individual modules for each assay that uses similar components (e.g., valves, heaters, metering chambers, mixers) installed on a polycarbonate base with a common reporter system. Assay miniaturization reduces the overall analysis time, increases accuracy by simultaneously identifying multiple targets, and enhances detector sensitivity by upconverting phosphor technology (UPT). Our microfluidic approach employs four interrelated components: (1) sample acquisition-OraSure UPlink™ collectors that pick-up and release bacteria, soluble analytes, and viruses from an oral sample; (2) microfluidic processing-movement of microliter volumes of analyte, target analyte extraction and amplification; (3) detection of analytes using UPT particles in a lateral flow system; and (4) software for processing the results. Ultimately, the oral-based microscale diagnostic system will detect viruses and bacteria, associated pathogen antigens and nucleic acids, and antibodies to these pathogens.",
keywords = "Confirmatory, Diagnostic, HIV, Lateral flow, Microfluidic, Multiplex analysis, Oral fluid, Pathogen, Point-of-care, Saliva",
author = "Abrams, {William R.} and Barber, {Cheryl A.} and Kurt McCann and Gary Tong and Zongyuan Chen and Mauk, {Michael G.} and Jing Wang and Alex Volkov and Pete Bourdelle and Corstjens, {Paul L A M} and Michel Zuiderwijk and Keith Kardos and Shang Li and Tanke, {Hans J.} and Niedbala, {R. Sam} and Daniel Malamud and Haim Bau",
year = "2007",
month = "3",
doi = "10.1196/annals.1384.020",
language = "English (US)",
isbn = "157331661X",
volume = "1098",
series = "Annals of the New York Academy of Sciences",
pages = "375--388",
booktitle = "Oral-based Diagnostics",

}

TY - GEN

T1 - Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT)

AU - Abrams, William R.

AU - Barber, Cheryl A.

AU - McCann, Kurt

AU - Tong, Gary

AU - Chen, Zongyuan

AU - Mauk, Michael G.

AU - Wang, Jing

AU - Volkov, Alex

AU - Bourdelle, Pete

AU - Corstjens, Paul L A M

AU - Zuiderwijk, Michel

AU - Kardos, Keith

AU - Li, Shang

AU - Tanke, Hans J.

AU - Niedbala, R. Sam

AU - Malamud, Daniel

AU - Bau, Haim

PY - 2007/3

Y1 - 2007/3

N2 - Confirmatory detection of diseases, such as HIV and HIV-associated pathogens in a rapid point-of-care (POC) diagnostic remains a goal for disease control, prevention, and therapy. If a sample could be analyzed onsite with a verified result, the individual could be counseled immediately and appropriate therapy initiated. Our group is focused on developing a microfluidic "lab-on-a-chip" that will simultaneously identify antigens, antibodies, RNA, and DNA using a single oral sample. The approach has been to design individual modules for each assay that uses similar components (e.g., valves, heaters, metering chambers, mixers) installed on a polycarbonate base with a common reporter system. Assay miniaturization reduces the overall analysis time, increases accuracy by simultaneously identifying multiple targets, and enhances detector sensitivity by upconverting phosphor technology (UPT). Our microfluidic approach employs four interrelated components: (1) sample acquisition-OraSure UPlink™ collectors that pick-up and release bacteria, soluble analytes, and viruses from an oral sample; (2) microfluidic processing-movement of microliter volumes of analyte, target analyte extraction and amplification; (3) detection of analytes using UPT particles in a lateral flow system; and (4) software for processing the results. Ultimately, the oral-based microscale diagnostic system will detect viruses and bacteria, associated pathogen antigens and nucleic acids, and antibodies to these pathogens.

AB - Confirmatory detection of diseases, such as HIV and HIV-associated pathogens in a rapid point-of-care (POC) diagnostic remains a goal for disease control, prevention, and therapy. If a sample could be analyzed onsite with a verified result, the individual could be counseled immediately and appropriate therapy initiated. Our group is focused on developing a microfluidic "lab-on-a-chip" that will simultaneously identify antigens, antibodies, RNA, and DNA using a single oral sample. The approach has been to design individual modules for each assay that uses similar components (e.g., valves, heaters, metering chambers, mixers) installed on a polycarbonate base with a common reporter system. Assay miniaturization reduces the overall analysis time, increases accuracy by simultaneously identifying multiple targets, and enhances detector sensitivity by upconverting phosphor technology (UPT). Our microfluidic approach employs four interrelated components: (1) sample acquisition-OraSure UPlink™ collectors that pick-up and release bacteria, soluble analytes, and viruses from an oral sample; (2) microfluidic processing-movement of microliter volumes of analyte, target analyte extraction and amplification; (3) detection of analytes using UPT particles in a lateral flow system; and (4) software for processing the results. Ultimately, the oral-based microscale diagnostic system will detect viruses and bacteria, associated pathogen antigens and nucleic acids, and antibodies to these pathogens.

KW - Confirmatory

KW - Diagnostic

KW - HIV

KW - Lateral flow

KW - Microfluidic

KW - Multiplex analysis

KW - Oral fluid

KW - Pathogen

KW - Point-of-care

KW - Saliva

UR - http://www.scopus.com/inward/record.url?scp=34248174353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248174353&partnerID=8YFLogxK

U2 - 10.1196/annals.1384.020

DO - 10.1196/annals.1384.020

M3 - Conference contribution

C2 - 17435143

AN - SCOPUS:34248174353

SN - 157331661X

SN - 9781573316613

VL - 1098

T3 - Annals of the New York Academy of Sciences

SP - 375

EP - 388

BT - Oral-based Diagnostics

ER -